柿子叶黄酮的提取工艺优选  

Research on Extraction of Flavone from Persimmon Leaves

作　　者：刁保忠[1] 靳维荣[1] 

机构地区：[1]聊城市人民医院制剂科,山东聊城252000

出　　处：《中国实验方剂学杂志》2012年第8期55-57,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省自然科学基金项目(ZR2009CQ025)

摘　　要：目的:研究柿子叶总黄酮的最佳提取工艺。方法:以芦丁、槲皮素和金丝桃苷的提取得率为指标,考察了提取次数、提取时间、料液比、乙醇体积分数对柿子叶黄酮提取率的影响,并通过正交试验设计确定了柿子叶的最佳提取工艺。结果:柿子叶黄酮的最佳提取工艺为:柿子叶中粉加入8倍量70%乙醇回流提取2次,每次1 h。结论:工艺验证结果显示该工艺稳定可靠,可以用于柿子叶黄酮的工业化提取。Objective：To determine the optimum extraction of flavone from Persimmon leaves.Method： The effects of extraction time and frequency,solid to liquid ratio,level of alcohol on the extraction rate of flavone was investigated with Rutosids,Meletin,Hyperoside as index;the optimum extraction of flavone from Persimmon leaves was determined by orthogonal experiment.Result： The optimum flavone from Persimmon leaves were： the extraction time was 1 h,the level of alcohol was 70%,solid to liquid ratio（g·mL）was 1∶ 8,and it was extracted two times.Conclusion： The optimum extraction process is achieved,suitable for industrialization.

关 键 词：柿子叶 芦丁 槲皮素 金丝桃苷 正交设计 

分 类 号：R283.6[医药卫生—中药学]