HPLC测定海南青牛胆中盐酸巴马汀的含量  

HPLC Determination of Palmatine Hydrochloride in Tinospora hainanensis

作　　者：吴丽媛[1] 张鹏威[2] 董琳[2] 刘明生 袁中文[1] 周郁斌[1] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]海南医学院药学院,海口571101 [3]海南省南药黎药研究院,海口571101

出　　处：《中国实验方剂学杂志》2012年第8期75-77,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2007BAI27B05)

摘　　要：目的:建立海南青牛胆药材中盐酸巴马汀的HPLC含量测定方法。方法:采用高效液相色谱法,Agilent TC-C18(4.6 mm×250 mm,5μm)柱,流动相为乙腈-0.4%三乙胺水溶液(24∶76),用磷酸调pH至4.0,流速1.0 mL.min-1,检测波长347 nm,柱温40℃。结果:盐酸巴马汀在线性范围0.006～0.15 mg(r=0.999 9)。平均回收率为99.8%,RSD 0.8%。结论:该测定方法精密度高,重复性好,可作为海南青牛胆中盐酸巴马汀的含量测定方法。Objective：To establish an HPLC method for determination of palmatine hydrochloride in Tinospora hainanensis.Method： HPLC method was applied on Agilent TC-C18 column（4.6 mm×250 mm,5 μm） at 40 ℃ with acetonitrile-0.4% triethylamine（24∶76）and adjusts pH with phosphoric acid to 4.0 as the mobile phase;detected wavelength was at 347 nm,and the flow rate was 1.0 mL·min-1.Result： The linear rage of palmatine chloride was 0.006-0.15 mg（r=0.999 9）.The average recovery was 99.8% and RSD was 0.8%.Conclusion： This determination method is accurate and reproducible,which can offer a suitable method for the determination of palmatine hydrochloride in T.hainanensis

关 键 词：海南青牛胆 盐酸巴马汀 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]