气相色谱法同时测定醒脑静注射液中麝香酮、龙脑、樟脑、异龙脑的含量  

Simultaneous Determination Muscone,Borneol, Camphor and Isoborneol in Xingnaojing Injection by GC

作　　者：方颖[1] 赵希贤[2] 赵鸣舒 龚卫红[3] 王庆纲[2] 孟大钧 

机构地区：[1]北京市药品检验所,北京100036 [2]北京市东城区药品检验所,北京100027 [3]北京中医药大学中药学院,北京100013

出　　处：《中国实验方剂学杂志》2012年第8期96-99,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立毛细管气相色谱法同时测定醒脑静注射液中麝香酮、龙脑、樟脑、异龙脑的含量。方法:采用气相色谱法,DB-FFAP(0.1μm×0.53 mm×30 m)毛细管色谱柱分离测定,FID检测器。进样口温度230℃。检测器温度240℃。用氮气作为载气,流速2.5 mL·min-1;色谱柱采用梯度升温,开始温度85℃,以5℃·min-1的升温速率升至185℃,以20℃·min-1的升温速率升至215℃,以1℃·min-1的升温速率升至230℃,保持5 min。分流比15∶1,进样量1μL,外标法定量。结果:麝香酮、龙脑、樟脑、异龙脑在同一色谱条件下获得分离,麝香酮、龙脑、樟脑、异龙脑在测定范围内呈良好线性关系,回收率符合要求。结论:方法简便、灵敏、准确,分离度、重复性好。Objective：To establish the simultaneous determination of muscone,borneol,camphor and isoborneol in Xingnaojing Injection.Method： The GC system consisted of capillary column,DB-FFAP-30M;FID as the detector.The column temperature was programmed from 85 ℃ to 185 ℃ at 5 ℃·min-1,then to 215 ℃ at 20 ℃·min-1,to 230 ℃ at 1 ℃·min-1（holding 5 minutes）.The inlet temperature was 230 ℃.The detect temperature was 240 ℃.Nitrogen was used as carrying gas（2.5 mL·min-1）.The split ratio was 15∶1;injection volume was 1 μL.The external standard method was employed.Result： Muscone,borneol,camphor and isoborneol in Xingnaojing Injection have been separated well under the same chromatographic condition.Muscone,borneol,camphor and isoborneol are in the determination of a good linear relationship within the scope.Recovery rate of the determination method all corresponded to the experimental standard.Conclusion： The method is simple,sensitive,accurate,separable and reproducible,and can be used to control the quality of Xingnaojing Injection.

关 键 词：醒脑静注射液 气相色谱法 麝香酮 龙脑 樟脑 异龙脑 

分 类 号：R284.1[医药卫生—中药学]