钩藤属植物部分品种药材钩藤碱成分的分析  

The Analysis of Rhynchophylline Contents in Part of Uncaria Schreb. Plant Breeds

作　　者：黄瑞松 张鹏 朱意麟[2] 严克俭[3] 覃冬杰 

机构地区：[1]广西民族医药研究院,南宁530001 [2]广西中医学院,南宁530001 [3]广西中医药研究院,南宁530022

出　　处：《中国实验方剂学杂志》2012年第8期125-128,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学基金项目应用基础研究专项(桂科基0991007);广西壮药质量标准研究项目(MZY2010074)

摘　　要：目的:探索钩藤属部分品种钩藤碱成分的含量,为评价这些品种药材质量提供试验依据。方法:应用HPLC测定钩藤属攀茎钩藤、侯钩藤、毛钩藤、倒挂金钩、北越钩藤和华钩藤33批不同产地药材钩藤碱含量。采用Gemini C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(含0.002 mol·L-1三乙胺,用冰乙酸调pH 7.5)(64∶36),流速为1.0 mL·min-1,检测波长254 nm。结果:攀茎钩藤和侯钩藤有5批样品可检出钩藤碱,但含量均较低,其余样品均未能检出钩藤碱。结论:试验所用钩藤属不同品种质量有所差异,同一品种质量随着产地不同而异。Objective：To probe rhynchophylline contents in part of Uncaria Schreb.plant breeds,and further provide experimental basis for evaluating the quality of medicinal materials.Method：Rhynchophylline in Uncaria Sandens,U.rhynchophylloides,U.hirsuta,U.lancifolia,U.homomalla and U.sinensis from 33 different producing areas were determined by HPLC.HPLC column was Gemini C18（4.6 mm×250 mm,5 μm）,mobile phase was methanol-0.002 mol·L-1 triethylomine（pH value 7.5,adjusted with acetic acid）（64∶36）,flow rate was 1.0 mL·min-1,and detection wavelength was 254 nm.Result： 5 batches of sample in U.Sandens and U.rhynchophylloides contained rhynchophylline,but content of rhynchophylline was low.The others do not contain rhynchophylline.Conclusion： The different breeds of Uncaria Schreb.plant vary in quality,while the quality of the same breed varies in different producing area.

关 键 词：钩藤属 品种 产地 钩藤碱 

分 类 号：R284.1[医药卫生—中药学]