黔产棘茎楤木抗炎作用及部分机制研究  

The Anti-inflammatory Effect and Mechanism of Aralia echinocaulis in Guizhou

作　　者：陈孟平[1] 乔为平[1] 乔晓彧[2] 莫艳珠 赵云华[1] 

机构地区：[1]贵阳中医学院,贵阳550002 [2]重庆医科大学,重庆400016

出　　处：《中国实验方剂学杂志》2012年第8期156-158,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵阳市科学技术项目([(2009)]筑科农合同字第800-9号);贵州省卫生厅科学技术基金项目

摘　　要：目的:研究黔产棘茎楤木的抗炎作用及部分机制。方法:采用二甲苯诱导小鼠耳肿胀、角叉菜胶诱导小鼠足爪肿胀、棉球诱导小鼠肉芽肿。弗氏完全佐剂诱导大鼠佐剂性关节炎(AA)模型:设正常对照组、模型对照组、阳性药对照组(雷公藤多苷片12 mg·kg-1)、黔产棘茎楤木高、中、低剂量组(剂量分别为3.5,1.75,0.88 g·kg-1);除正常对照组外,其余各组均采用弗氏完全佐剂足跖sc复制AA模型;各组于造模当天ig给药,连续35 d,采用硝酸还原法检测各组血清中一氧化氮(NO)含量;放免法检测各组血清中白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量。结果:黔棘茎楤木高、中剂量组对小鼠耳肿胀、足爪肿胀有明显抑制作用(P<0.01,P<0.05);对小鼠棉球肉芽肿亦有较好的抑制作用(P<0.05);并能降低AA大鼠血清中NO,IL-1β,TNF-α含量(P<0.01,P<0.05)。结论:黔棘茎楤木可能是通过影响炎性介质的释放和NO的生成而发挥抗炎作用的。Objective：To study the anti-inflammatory effect and mechanism of Aralia echinocaulis Hand-Mazzin produced in Guizhou.Method： Dimethylbenzene and carrageenin were used to,respectively,to cause ears edema in mice and paw swelling in rats;medical cotton and freund＇s complete adjuvant were used to,respectively,reduce granulomatous inflammation in mice and adjuvant arthritis（AA）in rats.Rats were randomly divided into control group,model group,positive drug group（tripterygium wilfordii 12 mg·kg-1）,A.echinocaulis high dosage group,middle dosage group,low dosage group（the dosage 3.5,1.75,0.88 g·kg-1）.The corresponding drugs were given at the day of modelling for 35 days.Nitric oxide（NO） content in serum was detected by nitrate reductase method and the level of interleukin-1β（IL-1β）,tumor necrosis factor-α（TNF-α） was measured with radioimmunoassay（RIA）.Result： High dosage group and middle dosage group of A.echinocaul were inhibited the ear edema in mice and swelling of paw（P0.01,P0.05）;meanwhile granuloma inflammation in mice was reduced（P0.05）,the level of NO,IL-1β and TNF-α was decreased（P0.01,P0.05）.Conclusion：A.echinocaul can restrain inflammatory reaction,the mechanism of which may be related to inhibiting secretion of NO,L-1β and TNF-α

关 键 词：黔棘茎楤木 抗炎 炎性介质 

分 类 号：R285.5[医药卫生—中药学]