兔急性肺血栓栓塞症血清TNF-α,IL-1β和IL-4的变化  

Changes of Serum TNF-α,IL-1β,IL-4 in Rabbit with Acute Pulmonary Thromboembolism

作　　者：姚艳敏[1] 徐彤彤[1] 武琦[1] 吕祥威[1] 

机构地区：[1]广西省桂林市桂林医学院附属医院特需病区,广西桂林541001

出　　处：《中国实验方剂学杂志》2012年第8期222-224,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2010年广西研究生教育创新计划项目(桂学位[2010]44号-559)

摘　　要：目的:监测兔急性肺血栓栓塞症(APTE)模型血清肿瘤坏死因子-α(TNF-α),(白细胞介素-4,1β)IL-4,IL-1β水平的变化,探讨炎症因子与肺损伤的关系。方法:自体血栓法建立兔APTE模型,随机分为对照组、模型组。ELISA法检测血清各指标,术毕行病理分析。结果:栓塞后IL-4在发病早期未见升高,发病后6 h明显升高,模型组与对照组分别为(42.8±1.35),(16.9±1.59)ng·mL-1(P<0.05),栓塞后4 hTNF-α模型组与对照组分别为(3.52±0.42),(1.29±0.36)ng·mL-1(P<0.05),栓塞后2 h IL-1β模型组与对照组分别为(2.66±0.12),(0.34±0.15)ng·mL-1(P<0.05),水平明显高于对照组。形态学观察结果可见,栓塞后肺动脉内血栓形成,肺组织萎缩、出血、炎性反应明显。结论:APTE模型血清炎症因子发生的不同变化,在引起急性肺损伤中起重要作用。Objective：To monitor changes in serum TNF-α,interleukin-4（IL-4） and interlukin-1β（IL-1β） in rabbit with acute pulmonary thromboembolism（APTE）,and to investigate the relation between inflammatory factors and lung injury.Method： Rabbit APTE model was established by auto-thrombus.The rabbits were randomly divided into control group and model group.ELISA was employed to determine serum TNF-α,IL-4 and IL-1β.Pathological analysis was carried out after the experimental operation.Result： In the early stage of APTE,there was no significant change in the parameters.After 6 h of thromboembolism,IL-4 was（42.8±1.35） ng·mL-1,whereas（16.9±1.59） ng·mL-1 in the controls（P0.05）.After 4 h and 2 h of thromboembolism,TNF-α and IL-1β were（3.52±0.42） ng·mL-1 and（2.66±0.12） ng·mL-1accordingly,whereas（1.29±0.36） ng·mL-1（P0.05） and（0.34±0.15） ng·mL-1（P0.05） in the controls.Pathological evidence showed that arterial thrombus,lung tissue atrophy,bleeding and inflammatory responses were significant.Conclusion： The inflammatory factors induced by APTE play important role in acute pulmonary lung injury.

关 键 词：肺血栓栓塞症 肿瘤坏死因子-α 白介素-4 白介素-1Β 

分 类 号：R285.5[医药卫生—中药学]