人类T型钙通道α1H和α1G基因在精索静脉曲张患者精子中的差异表达  

Decreased mRNA expressions of T-type channel α1H and α1G in the sperm of varicocele patients and their implication

作　　者：王世平[1] 王沛涛[2] 高峰[1] 李强[2] 刘忠强[2] 付秀艳[2] 李华琴[2] 王新生[2] 

机构地区：[1]莱芜市人民医院泌尿外科,山东莱芜271100 [2]青岛大学医学院附属医院泌尿男科学研究室,山东青岛266071

出　　处：《中华男科学杂志》2012年第4期331-334,共4页National Journal of Andrology

摘　　要：目的:探讨精索静脉曲张(VC)患者精子中T型钙通道α1H和α1G基因的表达与精索静脉曲张的关系。方法:根据WHO标准用计算机辅助精液分析(CASA)方法对所取精液进行检查,根据检查结果分为正常精液组(20例)、VC精液正常组(16例)、VC精液异常组(44例),用Percoll非连续梯度离心法对精液进行分离,应用半定量逆转录-聚合酶链反应(RT-PCR)技术分别检测3组精子T型钙通道α1H和α1G mRNA的表达。结果:与正常精液组相比较,VC精液正常组α1H和α1G mRNA的表达差异无显著性(P>0.05);VC精液异常组α1H和α1GmRNA的表达差异具有极显著性(P<0.01)。结论:T型钙通道α1H和α1G基因表达异常可能是导致VC患者精液质量下降从而不育的原因之一,此为VC不育的病因及治疗提供了新的方向。Objective： To study the relationship of varicocele（VC） with the expressions of T-type channel α1H and α1G in the sperm of VC patients.Methods： Based on the WHO criteria,we examined the semen samples by computer-aided sperm analysis（CASA）,and divided the samples into groups A（normal semen from volunteers,n = 20）,B（normal semen from VC patients,n = 16） and C（abnormal semen from VC patients,n = 44）.We optimized the semen by discontinuous Percoll grade centrifugation,and determined the mRNA expressions of T-type channel α1H and α1G in the three groups using using reverse transcription polymerase chain reaction（RT-PCR）.Results： Compared with group A,the mRNA expressions of α1H and α1G showed with no significant decrease in group B（P0.05）,but were remarkably reduced in group C（P0.01）.Conclusion： The abnormal mRNA expressions of T-type channel α1H and α1G may be one of the causes of declined semen quality and consequently infertility in VC patients,which has pointed out a new direction for the studies of the causes and treatment of VC-related infertility.

关 键 词：精索静脉曲张 T型钙通道 α1H α1G 逆转录-聚合酶链反应 

分 类 号：R698.2[医药卫生—泌尿科学]