干扰素及IL-6、IL-1β在肠道病毒71型感染过程中的作用  

Roles of interferon,interleukin-6 and interleukin-1β in enterovirus 71 infection

作　　者：范胜涛[1,2] 王丽春[2] 赵恒[2] 刘龙丁[2] 李琦涵[2] 

机构地区：[1]昆明医学院基础医学院,昆明650031 [2]中国医学科学院 北京协和医学院 医学生物学研究所,昆明650118

出　　处：《中国生物制品学杂志》2012年第4期441-445,共5页Chinese Journal of Biologicals

基　　金：国家重大科技专攻艾滋病和病毒性肝炎等重大传染病防治专项(2008ZX10004-014);国家重点基础研究973计划(2011CB504903)

摘　　要：目的探讨干扰素(Interferon,IFN)及白细胞介素-6(Interleukin-6,IL-6)、IL-1β在肠道病毒71型(Enterovirus 71,EV71)感染过程中的作用。方法采用EV71-FY23,在IFNα1b、IFNλ1和IFNγ分别存在的条件下感染Vero细胞及KMB17细胞,检测IFN对细胞的保护作用;建立EV71感染乳鼠模型,检测IFNα1b、IFNλ1、IFNγ、IL-6及IL-1β的抗病毒作用。结果IFNα1b和IFNλ1对EV71感染的细胞具有明显的保护作用,而IFNγ的保护作用不明显;IFNα1b对感染EV71的乳鼠具有较好的保护效果,其在病毒感染之前的预处理方式产生的保护效果较为明显;IL-1β对感染EV71的乳鼠亦有不同程度的保护作用,而IL-6预处理方式不但无保护作用,反而加速了乳鼠的死亡。结论 IFN和IL-1β对EV71感染具有保护作用,而IL-6无此作用。Objective To investigate the roles of interferon （IFN）, interleukin-6 （IL-6） and interleukin-ll5 （IL-1β） in enterovirus（EV）71 infection. Methods Vero and KMB17 cells were infected with EVT1-FY23 strain in presence of IFNcdb, IFNkl and IFNy separately, and the protective effects of IFNs on cells were observed. Suckling mouse model of EV71 infection was established, based on which the antiviral effects of IFNcdb, IFNkl, IFNy, IL-6 and IL-1β were observed. Results Both IFNc^lb and IFNX1 showed significantly protective effects on cells infected with EVT1, while IFN~/ showed no significantly protective effect. IFNα1b showed good protective effect on suckling mice infected with EV71, with which the pre-treatment before EV71 infection showed more significant effect. IL-115 also showed a certain protective effect on suckling mice infected with EV71. However, the pre- treatment with IL-6 showed no protective effect while accelerated the death of suckling mice. Conclusion IFN and IL-1β showed protective effect against EV71 infection, while IL-6 showed no protective effect.

关 键 词：干扰素 白细胞介素-6 白细胞介素-1Β 肠道病毒A型 人 

分 类 号：R373.2[医药卫生—病原生物学] Q511[医药卫生—基础医学]