超滤法去除A群脑膜炎球菌多糖料液中的苯酚  

Removal of phenol from group A meningococcal polysaccharide by ultrafiltration

作　　者：胡菁[1] 薛红刚[1] 郭蓉[1] 陈有喜[1] 瞿明霞[1] 陈浩军 

机构地区：[1]武汉生物制品研究所有限责任公司菌苗室,武汉430060 [2]Pall过滤器(北京)有限公司技术服务部,上海201203

出　　处：《中国生物制品学杂志》2012年第4期513-515,共3页Chinese Journal of Biologicals

摘　　要：目的采用超滤法去除A群脑膜炎球菌多糖料液中的苯酚。方法选取Omega OS010C10膜包(C型筛网,10 KD,过滤面积0.1 m2)和Omega OS010T12膜包(T型筛网,10 KD,过滤面积0.1 m2),分别对A群脑膜炎球菌多糖料液进行超滤,比较两种膜包不同压力对膜滤速的影响,测定超滤前后膜净水滤速的恢复率,分析经超滤和透析后样品中磷和苯酚含量及固体总量。结果 OS010T12膜包切向流速和压力的增加对滤速的影响较OS010C10膜包更显著,且在较低的压力差和过膜压力下,OS010T12膜包具有更高的过滤速度;在浓缩和洗滤阶段,OS010T12膜包的平均滤速较OS010C10膜包高约1.3倍;膜包经清洗后,膜净水滤速OS010T12膜包可恢复至试验前水平;两种膜包超滤的滤出液中苯酚的含量均符合《中国药典》三部(2010版)要求,且多糖回收率均高达90%以上,超滤用时比透析至少节约46 h,且耗水量只有透析工艺的20%。结论可用OS010T12膜包替换透析袋,对A群脑膜炎球菌多糖料液进行超滤,去除残余苯酚,为A群脑膜炎球菌多糖疫苗的生产提供了参考。Objective To remove phenol from group A meningococcal polysaccharide by ultrafiltration.Methods The materials of group A meningococcal polysaccharide were ultrafiltrated by using Omega OS010C10(type C screen,10 KD,filtration area: 0.1 m2) and Omega OS010T12(type T screen,10 KD,filtration area: 0.1 m2) membranes respectively,based on which the effects of various pressures on membrane filter velocity were compared,the recovery rates of membrane water purification filter velocities before and after ultrafiltration were determined,and the phosphorus,phenol and total solid contents in samples before and after ultrafiltration and dialysis were analyzed.Results As compared with those of OS101C10 membrane,the increased tangential velocity and pressure of OS010T12 membrane influenced the filtration rate significantly,and the filter velocity was high at low pressure difference and low transmembrane pressure.At steps of concentration and washing filter,the mean filter velocity of OS101T12 membrane increased by about 1.3 folds as compared with that of OS101C10 membrane.Conclusion The water purification filter velocity of OS101T12 membrane after washing recovered to the level before test.Both the phenol contents in ultrafiltrates by using the two membranes met the requirements in Chinese Pharmacopoeia(Volume Ⅲ,2010 edition),while the recovery rates of polysaccharide reached more than 90%.However,the time-consumed for ultrafiltration decreased by at least 46 h as compared with that for dialysis,while the water-consumed of the former was only 20% of that of the latter.

关 键 词：超滤 脑膜炎球菌多糖疫苗 苯酚 多糖 

分 类 号：R378.15[医药卫生—病原生物学] R392.33[医药卫生—基础医学]