大麦虫不同虫态甲醇粗提物化学成分的分析和比较  

Assay and comparison of chemical constituents of crude methanol extracts from different developmental stages of darkling beetle Zophpbas morio(Coleoptera:Tenebrionidae)

作　　者：陈雯婷[1] 刘海利[1] 姚励功[1] 叶柱良 郭跃伟[1] 

机构地区：[1]中国科学院上海药物研究所国家新药研究重点实验室,上海201203 [2]广东南方珍稀昆虫繁育研究中心,广东东莞523800

出　　处：《昆虫学报》2012年第3期357-360,共4页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(21021063);国家海洋"863"计划项目(2011AA09070102);欧盟第七框架计划IRSES项目(2010-2014);新药研究国家重点实验室基金(SIMM1105KF-04;SIMM1106KF-11)

摘　　要：为了探寻大麦虫Zophobas morio变态发育过程中化学成分的变化,本研究利用薄层色谱技术对大麦虫的3个虫态(幼虫、蛹和成虫)甲醇粗提物中的化学成分进行分析和比较,利用柱色谱技术、核磁共振氢谱技术(1HNMR)和气相色谱-质谱联用技术(GC-MS)对蛹甲醇粗提物进行重点分析。结果表明:虫蛹甲醇粗提物中含有幼虫和成虫甲醇粗提物中不存在的化学成分。对虫蛹甲醇粗提物进行针对性的结构研究,共鉴定出10个脂肪酸。其中,存在于大麦虫虫蛹中的10-十六碳酮酸、10-十八碳酮酸为首次从自然界昆虫中获得;(8E,11E)-8,11-十八碳烯酸、(9Z,12Z)-9,12-十八碳烯酸、(9Z,12Z,15Z)-9,12,15-十八碳烯酸、8-(3-辛基-2-环氧乙烷基)辛酸和8-(2-辛基环丙烷基)辛酸为首次从大麦虫中发现。以上研究结果为针对大麦虫不同生物阶段的开发利用提供新的理论依据。To study the chemical changes of darkling beetle Zophobas morio during metamorphosis,the crude methanol extracts of Z.morio in three developmental stages（larva,pupa and adult） were assayed by modern chromatographic techniques,1H NMR and GC-MS and compared.The results showed that pupae contained characteristic chemical constituents.The methanol extract of the pupae was systematically studied and ten fatty acids were identified.10-oxohexadecanoic acid and 10-oxooctadecanoic acid were found from pupae of Z.morio,which were separated from insects for the first time.（8E,11E）-8,11-octadecenoic acid,（9Z,12Z）-9,12-octadecenoic acid,（9Z,12Z,15Z）-9,12,15-octadecenoic acid,8-（3-octyl-2-oxiranyl）octanoic acid,and 8-（2-octylcyclopropyl）octanoic acid were also found from Z.morio for the first time.These results offer the new theoretical basis for the utilization of darkling beetle at different developmental stages.

关 键 词：大麦虫 蛹 甲醇粗提物 化学成分 10-十六碳酮酸 10-十八碳酮酸 

分 类 号：Q968[生物学—昆虫学]