天然牛黄与酶促牛黄HPLC-ELSD特征图谱比较  

HPLC-ELSD comparison on specific chromatogram of Calculus natural Calculus Bovis and Bovis with cultivated by glucuronidase

作　　者：李珂[1] 高允生[1] 李炳龙[1] 王福刚[1] 齐永秀[1] 

机构地区：[1]泰山医学院药学院,泰安271016

出　　处：《药物分析杂志》2012年第4期616-619,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：山东省高等学校科技计划(No.J10LF16);山东省科技发展计划(No.2011GGH22114)

摘　　要：目的:建立天然牛黄HPLC-ELSD特征图谱并与酶促牛黄特征图谱进行比较。方法:采用HPLC-ELSD法,色谱条件:Diamonsil C18柱(250 mm×4.6 mm,5μm);以甲醇和0.1%的醋酸水溶液为流动相,流速0.8 mL.min-1,梯度洗脱,漂移管温度110℃,气体流速2.0 L.min-1;分析时间60 min。结果:在选定的色谱条件下建立了天然牛黄色谱特征图谱共有模式,并对酶促牛黄与天然牛黄的特征图谱进行比较。结论:所建立的天然牛黄HPLC-ELSD特征图谱方法精密度高、重现性好,可用于酶促牛黄的鉴别研究。Objective：To establish an HPLC-ELSD specific chromatogram analysis method of natural Calculus Bovis with a comparative study of Calculus Bovis cultivated by glucuronidase.Methods：An HPLC-ELSD analysis was carried out on a Diamonsil C18 column（250 mm×4.6 mm,5 μm）with the mobile phase consisting of methanol（A）-0.1% acetic acid aqueous solution（B）in gradient mode.The flow rate was 0.8 mL·min-1.The temperature of drift tube was 110 ℃,flow rate of the gas was 2.0 L·min-1and the analytic time was 60 min.Results：Under the above chromatographic conditions,the mutual mode of HPLC specific chromatograms was set up,Calculus Bovis cultivated by glucuronidase had a similar specific chromatogram with the natural Calculus Bovis.Conclusion：The method is in good precision and reproducibility,which can be available for the identification of Calculus Bovis cultivated by glucuronidase.

关 键 词：天然牛黄 酶促牛黄 高效液相色谱法 蒸发光散射检测器 特征图谱 

分 类 号：R917[医药卫生—药物分析学]