Ⅰ型胶原海绵/血管内皮生长因子缓释系统的初步构建  

Construction of collagen type Ⅰ sponge/vascular endothelial growth factor slow-release material

作　　者：陈居铕[1] 李爱国[1] 陈鸿辉[1] 杨小红 谭见容 沈雁 张金丽 戴丽冰 

机构地区：[1]暨南大学附属广州市红十字会医院,广州510220 [2]广州市创伤外科研究所,广州510220

出　　处：《中国矫形外科杂志》2012年第8期738-743,共6页Orthopedic Journal of China

基　　金：广州市卫生局科研基金资助重点项目(编号:201102A212003);国家自然基金资助面上项目(编号:30973067)

摘　　要：[目的]研制Ⅰ型胶原(collagen type I,CI)海绵/血管内皮生长因子(vascular endothelial growth fac-tor,VEGF)缓释生物支架材料,了解该种材料中的VEGF体外缓释效果。为骨肌腱组织工程寻求一种新型复合支架材料。[方法]从CI海绵自身的力学、孔径、紫外最大吸收光谱、孔隙率、含水率、傅立叶红外光谱及与VEGF的组织相容性等方面研究VEGF胶原缓释系统支架材料的特性,利用生物学方法将VEGF负载于CI,制备具有VEGF缓释效果的复合支架。[结果]CI海绵能够成功的将VEGF包裹起来,可以达到明显的缓释作用,与VEGF有良好的组织相容性。[结论]CI海绵有理想的孔径和合理的生物力学及与VEGF良好的生物学相容性结构性质,VEGF可以从CI-VEGF缓释材料中缓慢释放,有明显的缓释效果,是一种具有良好材料特性的组织工程材料。[ Objective] To manufacture collagen typeI/VEGF （vascular endothelial growth factor, VEGF） slow-release timber biomaterial, investigate the slow-release effectiveness of this material, and find a new composition material for the tissueengineering of bone-tendon tissue. [ Method] This material was surveyed in respect with mechanic, aperture, uhraviolet absorp-ion maximum light spectrum, porosity, water content, fourier infrared spectrum,and tissue compatibility with VEGF, and utilized the biological method load the VEGF to collagen type Ⅰ, and the slow-release ability combined material was prepared. [ Resuit]The collagen type I sponge had the ability to encapulate the VEGF. And VEGF was slowly eleased from collagen type I sponge. This material had good biocompatibility with VEGF. [ Conclusion ] The collagen typeⅠ has desirable aperture and advisabie biomechanics. This material will be a good material for the bone-tendon tissue engineering.

关 键 词：组织工程 Ⅰ型胶原 血管内皮生长因子 

分 类 号：R318.08[医药卫生—生物医学工程]