乳品中金黄色葡萄球菌肠毒素B免疫传感检测方法的研究  

Research of an Electrochemical Immunosensor for Detecting Staphylococcus Aureus Enterotoxin B in Dairy Products

作　　者：高博[1] 孙秀兰[1] 张银志[2] 顾小红[2] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《分析科学学报》2012年第2期169-172,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.20806033);十二五科技支撑项目(No.201003008-08-03);张家港市科技局项目(No.ZKS0904);长三角联合攻关项目(No.09395811500)

摘　　要：本文利用自制的金黄色葡萄球菌肠毒素B(SEB)抗体构建了一种L-半胱氨酸和纳米金的双层自组装免疫传感器,采用循环伏安法及交流阻抗法对传感器进行表征与测定,并对各项相关条件进行优化,最终建立了SEB检测的标准曲线,线性范围分别在2～10 ng/mL和10～100 ng/mL,相关系数分别为0.9939和0.9926,检出限(S/N=3)为0.667 ng/mL,乳品检测回收率在84.3%～93.2%之间。该传感器特异性良好,稳定性好,可再生使用,可应用于乳品中SEB的快速检测。Staphylococcus aureus enterotoxin B（SEB） is a kind of extracellular toxins which is secreted by Staphylococcus aureus and mainly exists in high protein foods,such as milk.In this paper,we constructed an self-assembly immunosensor with self-made SEB antibody,double-layer L-cysteine and gold nanoparticle.The sensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy.We also optimized the experimeutal conditions,and established the SEB standard curve.The linear ranges were 2~10 ng/mL and 10~100 ng/mL with a correlation coeficient of 0.9939 and 0.9926,respectively,and the detection limit was 0.667 ng/mL（S/N=3）.The sensor shows good specificity,reusability and stability,and can be used in dairy products for the rapid detection of SEB.

关 键 词：免疫传感器 金黄色葡萄球菌肠毒素B L-半胱氨酸 纳米金 交流阻抗谱 

分 类 号：O657.1[理学—分析化学]