刚果红-孔雀石绿-左氧氟沙星分光光度法测定左氧氟沙星的含量  

Determination of Levofloxation with Congo Red-Malachite Green-Levofloxacin System by Spectrophotometry

作　　者：胡卫平[1] 王鑫[1] 董学芝[1] 张蕾[1] 焦嫚[1] 

机构地区：[1]河南大学化学化工学院环境与分析科学研究所,河南开封475004

出　　处：《分析科学学报》2012年第2期257-260,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.20875022);省部共建河南大学科研项目

摘　　要：在pH=3.55的NaAc-HAc缓冲溶液中,左氧氟沙星对刚果红(CR)和孔雀石绿(MG)反应生成的产物在可见光区有明显的作用,在618 nm波长处测定其吸收强度,结果表明体系的吸光度与左氧氟沙星的浓度在2～60μg.mL-1范围内呈现良好的线性关系,线性相关系数R=0.9905,检出限为0.053μg.mL-1。该方法可用于盐酸左氧氟沙星片剂、胶囊以及尿液中左氧氟沙星含量的测定。In a NaAc-HAc buffer solution（pH=3.55）,the absorbance of the chelate complex between congo red（CR） and malachite green（MG） was enhanced by levofloxacin（LVFX）,and a method for the determination of levofloxacin was therefore developed by spectrophotometric measurement at 618 nm.A good linearity in the range of 2~60 μg/mL and a detection limit of 0.053 μg/mL were obtained.The method has been applied to the determination of levofloxacin in tablets,capsules and urine samples and compared to the pharmacopoeia method with satisfactory results.

关 键 词：左氧氟沙星 刚果红 孔雀石绿 分光光度法 

分 类 号：O657.32[理学—分析化学]