氨酪酸与邻硝基酚的荷移反应研究  

Study on Charge Transfer Reaction of γ-Aminobutyric Acid with o-Nitrophenol

作　　者：韩玲军[1] 李省云[1] 安晓敏[1] 

机构地区：[1]太原师范学院化学系,山西太原030031

出　　处：《分析科学学报》2012年第2期285-287,共3页Journal of Analytical Science

基　　金：国家自然科学基金(21176020);山西省高校科技开发项目(20080025)

摘　　要：采用分光光度法研究了氨酪酸与邻硝基酚的荷移反应。由实验得知,用二甲亚砜做溶剂,室温下反应10 min后,可生成稳定的1∶1型的黄色络合物,其最大吸收波长λmax=446 nm,氨酪酸的浓度在2～16μg/mL的范围内遵循比尔定律,表观摩尔吸光系数ε=3.7×103L·mol-1·cm-1,桑德尔灵敏度S=0.028μg·cm-2。用拟定的方法测定氨酪酸片的含量,回收率在97.0%～102.7%范围内,相对标准偏差不大于2.1%。A simple,quick and sensitive spectrophotometric method has been established for the determination of γ-aminobutyric acid based on charge transfer（CT） reaction with o-nitrophenol in dimethyl sulfoxide solvent at room temperature.The composition of charge transfer complex of γ-aminobutyric acid with o-nitrophenol was found to be 1∶1 by Job＇s method.The apparent molar absorptivity of the complex was determined for 3.7×103 L·mol-1·cm-1,and the Sandell sensitivity was 0.028 μg·cm-2 at 446 nm.The calibration curve was linear in the concentration range of 2~16 μg/mL.This proposed method has been applied to the determination of γ-aminobutyric acid in tablets with satisfactory results.The recoveries were 97.0%~102.7% and RSDs were less than 2.1%.

关 键 词：邻硝基酚 氨酪酸 分光光度法 荷移反应 

分 类 号：O657.32[理学—分析化学]