中国南方汉族人群ApoC3基因启动子区-482C→T多态性与糖尿病肾病的相关性研究．  

The study of the association between ApoC3 gene promoter region-482C→T polymorphism and diabetic nephropathy

作　　者：陈波 宋滇平[1] 吴于滨[1] 石柔[1] 向茜 

机构地区：[1]昆明医学院第一附属医院糖尿病科,650032 [2]云南省第三人民医院肾内科

出　　处：《中国糖尿病杂志》2012年第4期263-266,共4页Chinese Journal of Diabetes

摘　　要：目的探讨载脂蛋白C3(ApoC3)基因启动子区-482C→T多态性与中国人DN的相关性。方法运用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP),在中国人群中对179例T2DM患者和60名正常对照者(NC)的ApoC3基因启动子区-482C→T的多态性进行检测,并比较各组间基因型频率和等位基因频率以及相关临床资料。结果 DN组(DN1+DN2)的T/T基因型频率及T等位基因频率高于DN0组(P<0.01),但DN2与ND1组间T/T基因型频率及T等位基因频率差异无统计学意义(P>0.05)。T2DM组中,T/T基因型组患者的血TG水平及24hUAlb均高于C/T和C/C基因型组者(P<0.01)。Logistic回归分析表明:ApoC3基因启动子区-482C→T多态性和DM病程与DN显著相关,-482T/T基因型是DN发生的危险因素;DN患者中,DM病程>5年是DN2发生的危险因素。结论在中国南方汉族人群中,ApoC3基因启动子区-482T/T基因型可能是DN发生的危险因素;病程>5年可能是DN进展的危险因素。Objective To investigate the association between ApoC3 gene promoter region insulin response element-482C→T polymorphism and diabetic nephropathy.Methods The polymorphism of ApoC3 gene promoter region-482C→T was detected by PCR-RFLP method in 179 unrelated Chinese with type 2 diabetes（DM group） and 60 normal controls（NC group）.The genotype frequency,allele frequency and relative clinical data were compared between groups.Ressults The frequencies of T/T genotype and T allele were significantly higher in DN（DN1＋DN2） group than in DN0 group（P〈0.01）.However,there were no significant differences in genotype between DN1 and DN2 groups（P〉0.05）.In DM（DN0＋DN1＋DN2） group,triglyceride（TG） and 24h urinary albuminuria（UAlb） were significantly higher in T/T genotype group than in C/T and C/C genotype groups（P〈0.01）.Logistic regression analysis showed that ApoC3 gene promoter region-482C→T polymorphism and diabetic duration were risk factors for DN occurrence.Conclusion The ApoC3 promoter region-482T/T genotype may be a risk factor of DN occurrence.Diabetic duration 〉5 years may be a risk factor for the progress of diabetic nephropathy in Chinese.

关 键 词：载脂蛋白C3基因 多态性 糖尿病肾病 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]