16S rDNA克隆文库法探索转基因香石竹对土壤细菌群落的影响  

Analysis of soil bacterial community composition by 16S rDNA clone library sampling from transgenic carnation

作　　者：白蓝[1] 赵明文[2] 贾军伟[1] 李鹏[1] 王金斌[1] 潘爱虎[1] 

机构地区：[1]上海市农业科学院生物技术研究所 上海市农业遗传育种重点实验室农业部转基因植物环境安全监督检验测试中心(上海),上海201106 [2]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《微生物学通报》2012年第4期435-447,共13页Microbiology China

基　　金：上海市科技兴农重点攻关项目(No.沪农科攻字2008第8-9号);转基因生物新品种培育重大专项课题(No.2008ZX08012-001)

摘　　要：【目的】通过研究转基因香石竹对土壤细菌群落的影响,为转基因香石竹的环境安全性评价提供依据。【方法】通过构建16S rDNA克隆文库,分析种植转基因和非转基因香石竹的土壤中细菌的群落结构组成。【结果】转基因和非转基因香石竹土壤中,共有的菌群有变形菌门(Proteobacteria)、浮霉菌门(Planctomycetes)、酸杆菌门(Acidobacteria),其中α-变形菌门、β-变形菌门、浮霉菌门为优势菌群;而在放线菌门(Actinobacteria)、疣微菌门(Verrucomicrobia)及未培养菌(Uncultured bacterium clone)等菌群存在部分差异。【结论】通过16S rDNA克隆文库方法揭示了转基因香石竹的土壤中细菌多样性十分丰富,其栽培对土壤细菌群落结构影响有限。[Objective] We did this research to analyze the impact on the soil bacterial popula-tion of the transgenic carnation,which laid the foundation for the safety assessment of GM carnation.[Methods] Bacterial 16S rDNA gene clone libraries of GM and non-GM carnations were constructed,and the soil bacterial populations of these carnations were compared.[Results] The results showed Alphaproteobacteria,Betaproteobacteria,Planctomycetes and Acidobacteria were shared with GM and non-GM carnation.Some differences were found in Actinobacteria,Verrucomicrobia and uncultured bacterium clone.[Conclusion] The results indicated that high bacterial diversity was found in these GM carnation soil bacterial libraries,and the cultivation of genetically modified carnation did not have a significant impact on the soil bacterial community structure.

关 键 词：16S rDNA克隆文库法 转基因香石竹 土壤细菌群落 

分 类 号：S154.3[农业科学—土壤学]