应用蛋白质组学的方法分析缺铁培养对化脓性链球菌MGAS5005的影响  

Proteomic analysis of effects of iron depletion on Streptococcus pyogenes MGAS5005

作　　者：吕洁[1] 余光创[1] 孙正华[1] 王南杰[1] 朱颖[1] 王红翠[1] 孙雪松[1] 

机构地区：[1]暨南大学生命与健康工程研究院,广东广州510632

出　　处：《微生物学通报》2012年第4期515-525,共11页Microbiology China

基　　金：国家自然科学基金项目(No.31000373);广东省自然科学基金项目(No.10451063201005247);暨南大学创新与培育项目(No.21611201)

摘　　要：【目的】研究铁缺失对化脓性链球菌的影响,并寻找摄铁系统中的关键蛋白。【方法】以化脓链球菌为模型,利用含Fe和不含Fe的培养基对细菌进行培养,收集全细胞蛋白进行双向电泳,定量软件分析电泳图谱,质谱鉴定差异蛋白,进而通过生物信息学分析蛋白上下游关系,从中找到关键蛋白。【结果】鉴定出20个差异蛋白,并用Cytoscape软件对差异蛋白相互关系网络进行节点分析找到其中5个瓶颈分子。【结论】在培养基中的Fe3+缺乏时,细菌的生物合成和含氮化合物、生物大分子等重要代谢受到很大影响,这为进一步阐明细菌铁代谢机制奠定了基础。[Objective] Analyze the effects of iron deficiency on Streptococcus pyogenes,and find the key proteins in the iron acquisition systems.[Methods] Streptococcus pyogenes was cultured in THY medium with or without iron.Total proteins were extracted for the 2DE-gel analysis.The altered proteins upon iron depletion were identified by mass spectrometry.Fur-ther bioinformatics analyzed the relationship between key differentially expressed proteins.[Results] The 20 alterated protein were identified.With Cytoscape analysis of the betweenness of these proteins,5 bottleneck proteins were detected.[Conclusion] The results showed that iron depletion affect the cellular biosynthetic processes,the metabolic process of compound containing nitrogen and the macromolecular metabolic process etc.This study laid a foundation for further studying the mechanisms of the iron metabolism in bacteria.

关 键 词：化脓性链球菌 金属蛋白 铁 蛋白质组学 

分 类 号：R373[医药卫生—病原生物学]