自体嗅粘膜联合β-七叶皂甙钠对脊髓损伤后FasL及Caspase-3表达的影响  

Effects of autologous olfactory mucoma combined with β-aesine sodium on expression of FasL and Caspase-3 after spinal injury

作　　者：徐年香[1] 刘曾旭[1] 罗小凤[1] 程华[1] 余庆[1] 刘卉芳[1] 刘德明[1] 

机构地区：[1]南昌大学基础医学院解剖学教研室,南昌330006

出　　处：《中国临床解剖学杂志》2012年第2期214-217,共4页Chinese Journal of Clinical Anatomy

基　　金：江西省教育厅课题(GJJ09097);江西省科技支撑项目(2009BSB09500)

摘　　要：目的探讨自体嗅粘膜联合β-七叶皂甙钠对Caspase-3及FasL表达与神经再生功能的影响。方法 SD大鼠随机分为损伤对照组(A组),自体嗅粘膜移植组(B组),自体嗅粘膜联合β-七叶皂甙钠组(C组),β-七叶皂甙钠组(D组)。每组分为1、3、7、14 d 4个时间点。制作脊髓半切模型,观察组织病理学改变;对动物后肢功能进行BBB评分;采用免疫组化法检测FasL、Caspase-3的表达。结果脊髓损伤后B、C、D组BBB评分均高于A组,FasL、Caspase-3阳性细胞数均较A组少,C组BBB评分较B、D组高,FasL、Caspase-3阳性细胞数较B、D组少。B、D组间无显著性差异。结论自体嗅粘膜与β-七叶皂甙钠两者之间有协同作用,可明显改善神经运动功能;此种保护作用可能与联合治疗能够抑制FasL、Caspase-3途径的细胞凋亡有关。Objective To approach the influence of autologous olfactory mucoma combined with β-aesine sodium on expression of FasL and Caspase-3 in injuried spinal cord. Methods Sprague-Dawley rats with spinal T10 hemisection were divided into four groups as follows： control group（group A）,autologous olfactory mucoma transplantation group（group B）,combination group（group C） and β-aesine sodium group（group D）.Each group was subdivided into four groups： 1,3,7 and 14d.for evaluating pathological changes of the spinal cord,the Basso-Beattie-Bresnahan（BBB） scales of hindlimb movement function were carried out.The expression of FasL and Caspase-3 in injured spinal cord were detected by immunohistochemistry analysis.Results BBB scores of group B,C and D were higher than group A.FasL and Caspase-3 positive cells in group B,C and D were less than group A.BBB scores of group C were higher than group B and D,and FasL and Caspase-3 positive cells of group C were less than group B and D.There was no significant difference between group B and D.Conclusion Synergistic effects occurs for promoting the recovery of hindlimb motor function after spinal injury combined autologous olfactory mucoma with β-aesine sodium,which also help to restrain apoptosis by reducing expression level of FasL and Caspase-3.

关 键 词：脊髓损伤 自体嗅粘膜 Β-七叶皂甙钠 CASPASE-3 FASL 

分 类 号：R324[医药卫生—人体解剖和组织胚胎学]