红叶石楠离体植株再生频率的影响因素研究  

Factors that Influence in vitro Plant Regeneration of Photinia fraseri

作　　者：方敏彦[1] 梁慧敏[1] 夏阳 燕丽萍 刘翠兰 

机构地区：[1]江苏农林职业技术学院,江苏句容212400 [2]山东林业科学研究所,济南250014

出　　处：《西北植物学报》2012年第2期403-408,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：江苏省高技术研究项目(BG2007317)

摘　　要：以红叶石楠带芽茎段及叶片为外植体,分析激素和培养条件等因子对愈伤组织诱导及植株再生的影响。结果表明,MS+0.10mg/L 2,4-D+0.50mg/L NAA+0.50mg/L 6-BA+0.50mg/L KT为最佳愈伤组织诱导培养基,暗培养的愈伤组织诱导率高于光培养,其愈伤组织诱导率可达100%(带芽茎段)和98%(叶片)。MS+0.50mg/L IBA+2.00mg/L 6-BA+2.00mg/L KT为最佳分化增殖培养基,分化率91%以上,增殖倍数6.8以上,均达到最高。1/2MS+0.50mg/L IBA+0.01mg/L NAA为最佳生根培养基,生根率92%,生根量4.4根/株,均达到最高。The bud and leaf of Photinia fraseri were used to study the effect of hormone and cultivation condition on callus induction and plantlet regeneration. The results indicated that the best medium for cal- lus induction was MS4＋O.10mg/L 2,4-D＋0.50 mg/L NAA＋0.50 mg/L 6-BA＋0.50 mg/L KT. The callus induction rate under dark culture kept higher than that of light culture. The callus induction rates were 100 % （bud） and 98 % （leaf）, respectively. The best medium for proliferation was MS ＋0.50 mg/L IBA ＋ 2.00 mg/L 6-BA-b2.00 mg/L KT. The differentiation rate was more than 91% and multiplication times were more than 6.8. The best medium for roots formation was 1/2MS＋0. 50 mg/L IBA＋0. 01 mg/L NAA,and the rooting rate was 90.0%. The rooting number was 4.4 per plant.

关 键 词：红叶石楠 愈伤组织 植株再生 

分 类 号：Q813.1[生物学—生物工程]