普通小麦离体胚组织的脱分化和再分化培养特性  

Studies on Dedifferentiation and Re-differentiation Characteristics of in vitro Embryos of Common Wheat

作　　者：杨亚辉[1] 陈耀锋[1] 李春莲[1] 薛芳[1] 安强[1] 周文丽[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《西北农业学报》2012年第2期26-30,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家转基因生物新品种培育项目(2008ZX08002003;2009ZX08002008B);陕西省13115重大科技专项(2009ZDKG-11);唐仲英育种项目(A212020908);陕西省科技攻关项目(2011K02-01)

摘　　要：以西农1376和小偃22两个普通小麦品种为材料,对授粉后不同时间段幼胚进行脱分化、再分化培养,分析不同胚龄的小麦离体胚培养特性。结果表明,胚龄22d时,西农1376和小偃22胚诱导形成的愈伤组织频率最高,胚龄26d胚发芽率最高,胚龄16d愈伤组织再分化率最高。在小麦离体胚组织培养中,胚龄是影响小麦胚离体培养特性的关键因素。The in vitro culture characteristics of wheat immature embryos in different developmental stages were systematically studied through the dedifferentiation and re-differentiation.Immature embryos of different developmental stages of two wheat cultivars,Xinong 1376 and Xiaoyan 22 were used as the materials,the culture characteristics were statistically analyzed through dedifferentiation and re-differentiation.The results showed that the callus induction frequencies of Xinong 1376 and Xiaoyan 22 were the highest for the embryos of 22 days after pollination;for the embryos of 26 days,the germination rate was the highest;for the 16 days,the callus re-differentiation was the highest.The age of embryo was the key factor which affected the in vitro embryo culture characteristics of wheat.

关 键 词：胚龄 脱分化 再分化 

分 类 号：S512.1[农业科学—作物学]