矽尘接触人群血清蛋白质指纹与接尘量的相关性分析  

Correlation analysis between the exposure levels and the serum protein fingerprints in population exposure to silica

作　　者：孔海霞[1] 刘伟[2] 马庆波[3] 陈娟娟[4] 魏茂提[5] 王世鑫[4] 翁亚光[1] 

机构地区：[1]重庆医科大学医学检验系临床检验诊断学省部共建教育部重点实验室,400016 [2]天津市传染病医院检验科天津肝病研究所 [3]安康职业技术学院 [4]天津市东丽医院 [5]武警医学院部队流行病学与统计学教研室

出　　处：《中华劳动卫生职业病杂志》2012年第4期258-260,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金面上项目（30771788）;天津市卫生局科技基金（06KG10）;天津市应用基础研究计划面上项目（06YFJMJC09900）

摘　　要：目的筛选矽尘接触人群血清蛋白质指纹并分析其与接尘量的相关性。方法应用液体芯片飞行时间质谱技术检测Ⅰ期矽肺组（25例）、正常对照组（30例）、矽尘接触组（30例）及可疑矽肺组（30例）血清蛋白质指纹并筛选出差异显著的蛋白，对每个样品差异蛋白的峰强度与其对应的接尘量进行相关性分析。结果4组间共筛选出5个差异显著蛋白，其中5081Da、5066Da为表达上调蛋白，3954Da、2021Da、1777Da为表达下调蛋白，将这些蛋白峰强度分别与其相应的接尘量进行相关分析，未发现蛋白峰程度与接尘量有相关关系（FI=0．039，r2=0．104，r3=-0．047，r4=-0．028，r5=-0．016，P〉0．05）。结论本次研究中矽尘接触人群血清蛋白质指纹与接尘量不存在相关关系。Objective To explore the correlation between the exposure levels and serum protein finger- prints in population exposed to silica. Methods Liquid chip time-of-flight mass spectrometry technology was used to investigate the serum profiles in control group （30 cases）, group exposed to silica （30 cases）, silicosis group （I stage, 25 cases） and suspected silicosis group （30 cases）, and screen the differential expression proteins. The correlation between the levels of the differential expression proteins and the exposure levels was per- formed. Results Five differential expression proteins were found among 4 groups, the expression of 5081 and 5066 proteins was upregulated, and the expression of 3954, 2021 and 1777 proteins was downregulated. There was no the correlation between the exposure levels and the peak with M/Z among those proteins. Conclusion the resuhs of present investigation indicated there was no correlation between thw exposure levels and protein/ peptide peak.

关 键 词：粉尘 光谱分析 质量 

分 类 号：R135.2[医药卫生—劳动卫生]