老龄肾阳虚证差异表达基因的筛选与鉴定  

Differentiation and Identification of Expressed Genes of Aged kidney-Yang Deficiency Syndrome

作　　者：李炜弘[1] 许嗣立[1] 张天娥[1] 丁维俊[1] 黄禹峰[1] 曾跃琴[1] 谭雪菊[1] 

机构地区：[1]成都中医药大学中医诊断学教研室,四川成都610075

出　　处：《辽宁中医杂志》2012年第4期590-592,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(30600795;30973690)

摘　　要：目的:筛选并鉴定与老龄肾阳虚证相关的差异表达基因。方法:从前期基因表达谱芯片初步获得的差异表达基因中筛选出25个基因,选取18例肾阳虚证患者和6例正常对照者,采用半定量RT-PCR检测其血液中这25个基因的表达情况,从中鉴定出与老龄肾阳虚证相关的差异表达基因。结果:有8个基因在18例老龄肾阳虚证患者和6例正常人间的表达水平存在明显差异,这8个基因主要涉及细胞周期和凋亡,肿瘤发生以及免疫。结论:老龄肾阳虚证的发生涉及一系列免疫紊乱的复杂过程,推测肾阳虚可能与肿瘤的发生存在某种程度的关联。Objective：To filter and verify the relevance of the differentially expressed genes of aged kidney-Yang deficiency syndrome.Methods：From the earlier gene expression profile chip,we filtered 25 genes in the differentially expressed genes,18 elderly patients with kidney-Yang deficiency and 6 healthy people were selected,and the semi-quantitative skill of the RT-PCR was used to detect the 25 genes＇expressions in the blood so as to verify the differentially expressed genes which are correlated with the aged kidney-Yang deficiency syndrome.Results：There are 8 genes that have the significant differences in expression between the 18 elderly patients with kidney-Yang deficiency and the 6 healthy people.The 8 genes mainly involve the mitotic cycle and apoptosis,cancer and immunity.Conclusion：The aged kidney-Yang deficiency syndrome is related to dysfunction of the immune system.Hence,we can conjecture some relationship between the kidney-Yang deficiency syndrome and the development of tumor.

关 键 词：肾阳虚证 差异表达基因 RT-PCR 肿瘤 

分 类 号：R596.2[医药卫生—内科学]