控制大白菜和白菜型油菜叶缘裂刻的QTL定位及分析  

QTL Mapping of a Leaf-lobed Trait in Brassica campestris

作　　者：邓杰[1] 王辉[1] 程锋[1] 武剑[1] 王晓武[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《园艺学报》2012年第4期661-668,共8页Acta Horticulturae Sinica

基　　金：国际科技合作项目(2010DFA31730);国家自然科学基金项目(30800753);中央级公益性科研院所基本科研业务费专项(201011)

摘　　要：利用叶缘光滑无裂刻的大白菜[Brassica campestris L.ssp.pekinensis（Lour）Olsson]材料‘Z16’和叶缘深裂的白菜型油菜（Brassica campestris L.var.yellow sarson Prain）‘Yellow Sarson143’构建的120个株系的BC2DH群体及已构建的包含10个连锁群的遗传图谱,利用JoinMap4软件及MQM作图法,对叶缘裂刻性状进行QTL定位分析。3次重复试验中,分别在第3、10号染色体上的相同位置各检测到1个控制叶缘裂刻性状的QTL,具有重复性。各QTL的LOD值在7.66－18.99间,可解释26.4%~44.7%的表型变异。通过大白菜与拟南芥注释基因比对,生物活性赤霉素（GA1、GA4）合成的关键基因BrGA20ox3位于第10染色体的QTL区域,对143施加外源赤霉素GA3能明显抑制叶缘裂刻表型,因此BrGA20ox3为控制叶片裂刻的可能候选基因。对BrGA20ox3克隆测序获得与该基因共分离的特异标记（BrIDlobe-2）,对进一步研究叶缘裂刻性状及分子标记辅助育种具有重要的意义。The objective of this study was to map and analyze QTLs controlling leaf-lobed trait in the population constructed by Chinese cabbage‘Z16’[Brassica campestris L. ssp. pekinensis（Lour）Olsson] and‘Yellow Sarson 143’（Brassica campestris L. var. yellow sarson Prain）,so as to provide information for molecular marker assisted selection in Chinese cabbage breeding. Leaf-lobed trait was detected in parents and individuals of BC2DH population of Z16 × 143 in different growing seasons. An integrated map of 559.08 cM with 10 linkage groups was also used for the QTL analysis. Using the genetic map and multiple QTL model（MQM）method of software package MapQTL（version 4.0）,QTLs were detected in the same position on 3rd and 10th chromosomes in the three different growing seasons. The LOD values varied from 7.66 to 18.99 which explained 26.4% - 44.7% of the phenotypic variation. When analyzed with annotated genes between Arabidopsis thaliana and B. rapa in the QTL region on 10th chromosome,one active GA biosynthetic gene was found as the candidate gene for the leaf-lobed trait. The lobed phenotype was inhibited with application of exogenous GA3 in line 143.

关 键 词：大白菜 白菜 裂刻 QTL BrGA20ox3 

分 类 号：S634.1[农业科学—蔬菜学] S634.3[农业科学—园艺学]