富铬酵母对糖尿病模型小鼠血脂及胰腺的影响  

Effect of high chromium yeast on blood lipids and pancreatic islets in type 2 diabetic mice model

作　　者：刘鹭[1] 金文[2] 吕加平[1] 

机构地区：[1]中国农业科学院农产品加工研究所农业部农产品加工与质量控制重点开放实验室,北京100193 [2]中国医学科学院药用植物研究所,北京100193

出　　处：《中华临床营养杂志》2012年第2期93-98,I0001,共7页Chinese Journal of Clinical Nutrition

基　　金：国家航天育种工程项目（2006HT20013）

摘　　要：目的研究富铬酵母对小鼠Ⅱ型糖尿病的影响。方法共60只Balb／C小鼠进入本研究。未注射链脲霉素的10只Balb／C小鼠为正常组，其余50只小鼠通过小剂量注射链脲霉素建立Ⅱ型糖尿病模型。按照血糖值平均分为5组，其中二甲双胍为阳性对照药物组，普通酵母为对照组，富铬酵母饲喂小鼠分为低[250μg／（kg·d），以铬元素计]、中[500μg／（kg·d）]、高[1000tLg／（kg·d）]3个剂量组，灌胃饲养15周。测定体重和空腹血糖值、糖耐量值等血液生化指标，进行胰腺病理组织分析。结果与对照组比较，富铬酵母中剂量组的甘油三酯显著降低（P=0．043），低、中剂量组的总胆固醇显著降低（P=0．006、P=0．003），低、高剂量组的血清胰岛素浓度显著提高（P=0．011、P=0．002），高剂量组的糖基化血红蛋白显著下降（P=0．027）。胰岛组织病理检查显示富铬酵母中、高剂量组未出现炎性细胞浸润现象。结论富铬酵母菌体对小鼠糖尿病有一定的改善作用，但本研究未设有机铬对照组，需进一步对比研究。Objective To study the impact of high chromium yeast on type 2 diabetic mice. Methods A total of 60 Balb/C mice were used in this study. Fifty mice were injected with streptozotocin to induce type 2 diabetes, while the ten mice without injection were in the normal group. The type 2 diabetic mice were divided into 5 groups according to their fasting blood sugar levels, including 3 groups receiving high chromium yeast at low [ 250 μg/ （kg · d） ], medium [500 μg/ （kg · d） ], and high [1000 μg/ （ks · d） ] doses, 1 group receiving metformin （ positive control）, and 1 group receiving normal yeast （ negative control）. The yeast or metformin were given through by gastric lavage daily for 15 weeks. Body mass and blood biochemical indexes （fasting blood glucose, glucose tolerance, etc） were determined, and the pathological examination of pancreas was conducted. Results Compared with negative control group, medium dose high chromium yeast group had significantly lower triglyceride （ P = 0. 043 ）, low and medium dose groups had lower total cholesterol （ P = 0. 006, P = 0. 003 ）, low and high dose groups had higher insulin levels （ P = 0. 011, P = 0. 002 ）, and high dose group had significantly reduced glycosylated hemoglobin （P = 0. 027 ）. Pathological examination of pancreatic islets revealed that medium and high dose groups did not develop infiltration of inflammatory cells. Conclusions High chromium yeast may improve type 2 diabetes mellitus. Further studies are needed to confirm the conclusion of this study with an organic chromium control group.

关 键 词：富铬酵母 小鼠Ⅱ型糖尿病模型 胰腺 葡萄糖耐量因子 

分 类 号：R587.1[医药卫生—内分泌]