上海地区妇女宫颈脱落细胞中人乳头瘤病毒型别分布特征  

The distribution characteristics and analysis of subtypes of human papilloma virus of women cervical exfoliated cells in Shanghai region

作　　者：李江燕[1] 陈洁[1] 侯晓菁[1] 仲人前[1] 王皓[1] 

机构地区：[1]第二军医大学附属长征医院实验诊断科,上海200003

出　　处：《现代免疫学》2012年第2期148-151,共4页Current Immunology

摘　　要：了解上海地区妇女宫颈人乳头瘤病毒(HPV)感染的阳性率、亚型及感染分布情况,为预防HPV感染和宫颈癌提供理论依据。采用导流杂交技术对2076例来院就诊妇女宫颈脱落细胞进行21种HPV基因亚型检测,分析HPV感染高危因素及亚型分布特点。结果:在被调查的2076例妇女中,HPV感染者为593例,阳性检出率28.6%,21种基因型中只有43型没被检出;在检出类型中单一感染394例,占阳性百分比为66.4%;多重感染199例,占阳性百分比为33.6%(其中双重感染127例、三重感染44例、四重感染19例、五重感染3例、六重感染4例,八重感染2例)。检测结果中高危基因型排在前五位的是HPV52(7.13%)、HPV16(5.83%)、HPV58(4.91%)、HPV53(3.28%)、HPV18(2.50%),低危基因型主要是HPV11(2.75%)、HPVCP8304(2.75%)。不同年龄段的人群HPV阳性率不同,其中18～25岁的人群检出率最高,达到了38.3%。本地区就诊妇女宫颈HPV感染率较高,主要为单一基因型感染,常见的HPV感染基因型为HPV52、16、58、53、18、6、11、cp8304,与中国其他地区的分布特点不同,符合HPV感染具有地域性的特点。上海地区HPV感染的主要基因型与目前现有的HPV四价疫苗不尽相同,提示需要研制与本地区主要基因型高度相关的多价疫苗来预防宫颈癌的发生。To investigate the distribution of female cervical human papilloma virus （HPV） infection in Shanghai, and provide a theoretical basis for the prevention of HPV infection and cervical cancer, 2076 specimens of female cervical exfoliative cell were collected and tested for 21 kinds of HPV subtypes by DNA flow-through hybridization technique and analyzed for high-risk fac- tors of HPV infection and distribution characteristics of HPV subtypes. Among the 2076 women investigated, 593 cases were HPV infection, the positive detected rate is 28.6~ ,only type 43 was not detected among the 21 kinds of gene types; 394 cases were single infection, the positive percentage was 66.4%; 199 cases were multiplicity infection, the positive percentage was 33. 6% （ among them, 127 cases were dual infection, 44 cases were triple infection; 19 cases were four re-infection, 3 cases were five re-infection, 4 cases were six reinfection,2 cases were eight re-infection ）; In the detected results, the top five high-risk gene types were HPV52（7.13%）, HPV16（5.83%）, HPV58（4.91%）, HPV53（3.28%）, HPV18（2.50%） ;Low-risk gene types were mainly HPVll（2.75%） , HPVCP8304（2.75%）. HPV positive rates were different in different age groups, which the HPV positive rate in the age group of 18- 25 years old was the highest（38.3%）. The women in Shanghai have high inci- dence of HPV infection, and the majority of cases are a re-infection. The infection distribution of HPV sub-types are mainly HPV52, 16, 58, 53, 18, 6, 11, CP8304 in Shanghai region, which is different from those of other regions in China, conforming to the regional characteristic of HPV infection. But the major genotypes of HPV infection are different from the quadriva- lent vaccine available. In summary, a new vaccine need to be developed,which is suitable to the distribution characteristics of HPV subtypes and may prevent cervical cancer in Shanghai region.

关 键 词：人乳头瘤病毒 宫颈癌 导流杂交技术 

分 类 号：R446.5[医药卫生—诊断学]