联合检测抗α-胞衬蛋白、抗SSA和抗SSB抗体在干燥综合征诊断中的应用  

Application of combined detection of anti-α-fodrin antibody,anti-SSA and anti-SSB in diagnosis of Sjgren's syndrome

作　　者：蔡逸婷[1] 刘庆中[1] 赵超[1] 李莉[1] 

机构地区：[1]上海交通大学附属第一人民医院检验科,上海200080

出　　处：《现代免疫学》2012年第2期152-155,共4页Current Immunology

摘　　要：探讨抗α-胞衬蛋白、抗SSA(Ro60)和抗SSB抗体的检测对干燥综合征的诊断价值。酶联免疫吸附试验(ELISA)检测40例原发性干燥综合征、20例继发性干燥综合征、60例其他结缔组织病和120例健康人血清中抗SSA(Ro60)抗体、抗SSB抗体和抗α-胞衬蛋白抗体,同时记录患者的临床资料。结果:抗SSA(Ro60)、抗SSB和抗α-胞衬蛋白抗体检测诊断干燥综合征(Sjgrens syndrome,SS)的敏感性分别为65%、48%和63%;特异性分别为92%、94%和90%。SS患者抗α-胞衬蛋白抗体阳性率(63%)显著高于非干燥综合征患者(27%,P<0.05)。抗SSA(Ro60)和抗SSB抗体均阴性的SS患者抗α-胞衬蛋白抗体阳性率为65%(13/20)。ELISA方法联合同时检测三种抗体对SS敏感性和特异性分别为88%和90%,与三种抗体单独检测相比特异性相近而敏感性显著提高(P<0.05)。抗α-胞衬蛋白抗体检测对SS诊断敏感性高,对抗SSA抗体和抗SSB抗体均阴性的患者联合检测三种抗体可显著提高SS诊断的敏感性,检测抗SSA和抗SSB同时也需要检测抗α胞衬蛋白抗体。To study the significance of combined detection of anti-α-fodrin antibody, anti-SSA antibody and anti-SSB antibody in diagnosing Sjogren＇s syndrome（SS）, using ELISA to detect the anti-α-fodrin antibody, anti-SSA antibody and anti-SSB antibody in 3 groups of patients：40 patients with primary Sjogren＇s syndrome, 20 with secondary Sjogren＇ s syndrome, 60 with other connective tissue diseases, and 120 healthy people as normal control. The clinical data of the patients were collected. The results showed the sensitivity of anti-α-fodrin antibody, anti-SSA antibody and anti-SSB antibody to detecting Sjogren＇s syndrome was 65%, 48% and 63% respectively, and the specificity was 92%, 94% and 90% respectively. The positive rate of anti-α-fodrin antibody（63%） in SS was significantly higher than that in non-SS（27 %, P〈0.05）. The positive rate of anti-α-fodrin antibody was 65 % （13/20） in those SS with negative anti-SSA antibody and negative anti-SSB antibody. The sensitivity and specificity of combined detection of anti-α-fodrin antibody, anti-SSA antibody and anti-SSB antibody in SS were 88 % and 90% respectively. Compared with detecting single antibody, combined detection had similar specificity but significantly improved sensitivity （P〈0.05）. The detection of anti-α-fodrin antibody has higher sensitivity in diagnosing SS. Giving combined detection of all three antibodies to those patients with negative anti-SSA antibody and anti-SSB antibody can significantly improve the diagnosis of SS. Therefore it may be necessary to detect anti-α-fodrin antibody in addition to detect anti-SSA antibody and anti-SSB antibody.

关 键 词：抗Α-胞衬蛋白抗体 抗SSA抗体 抗SSB抗体 干燥综合征 酶联免疫吸附试验 

分 类 号：R593.2[医药卫生—内科学]