小鼠胎肝红系细胞在分化过程中形态学观察及时序性表达分析  

The Morphological Observation and Time-dependent Changes Analysis of Erythroblasts during Differentiation in Mouse Fetal Livers

作　　者：郑雅娟[1] 陈涛[1] 杨祖立[1] 曹玲玲[1] 毛群铨[1] 薛建有[1] 吴业卿[1] 张世馥[1] 

机构地区：[1]浙江理工大学生命科学学院蛋白质组学与分子酶学实验室,杭州310018

出　　处：《中国细胞生物学学报》2012年第4期317-324,共8页Chinese Journal of Cell Biology

基　　金：浙江理工大学研究生创新基金(No.YCX-S11019)资助项目~~

摘　　要：小鼠胎肝是小鼠发育早期主要的造血器官，红系细胞在胎肝造血过程中形态特征和组成成分等方面发生了明显变化。根据红系细胞体积的变化，利用Countstar细胞计数仪对小鼠E12．5-E17．5胎肝中直径8-14岬细胞进行数量统计，再结合观测到的红系细胞的形态特征和血红蛋白表达量的不同，将E9．5．E17．5胎肝中的细胞分为10类。统计结果显示，随着胎肝造血系统的发育，哺乳类红系细胞在终末分化时出现细胞体积减小、细胞核固缩、排核和血红蛋白表达量增加等时序性变化。红系细胞表面特异标志Terll9和CD71在EryD中高表达而在成体骨髓细胞和外周血细胞中表达较低的结果表明胎肝中红系细胞具有较高的分化能力。这些数据为研究红系分化、克隆红系分化相关基因及探讨红白血病发生的机制提供了理论依据。Mouse fetal liver is an important early-stage blood-forming organ. During hematopoiesis, the morphology change of erythroblasts is significant. In view of the volume change of erythroblasts, the quantity of the diameter 8 μm to 14 μm erythroblasts from mouse EI2.5-EI7.5 fetal liver was measured by Count star cytom- eter. The erythroblasts from mouse E12.5-E17.5 fetal liver were divided into ten groups based on their morphology and hemoglobin expression. This study showed that with the development of fetal liver hematopoietic system, cell volume reduction, nuclear condensation, denucleation and hemoglobin up-regulation as well as time-dependent changes occurred during the terminal differentiation of mammalian erythroblasts. Quantitative immunocytochemi- cal analysis of erythro-specific marker Terl 19 and CD71 further confirmed that the differentiation capability of fetal liver erythroblasts was higher than that of adult bone marrow cells and peripheral blood cells. These data together provided theoretical support to investigate the mechanisms of erythroid differentiation and erythroleukemia.

关 键 词：胎肝 红系细胞 时序性分化 免疫细胞化学 

分 类 号：Q24[生物学—细胞生物学]