蝴蝶兰合子和胚的分离  

Isolation of Zygotes and Embryos in Phalaenopsis

作　　者：伍成厚[1,2] 李冬妹[3] 田惠桥[1] 

机构地区：[1]厦门大学生命科学学院,厦门361005 [2]广州市园林科学研究所,广州510405 [3]顺德职业技术学院,顺德528300

出　　处：《中国细胞生物学学报》2012年第4期361-365,共5页Chinese Journal of Cell Biology

基　　金：广东省科技计划项目(No.2007B020801007);广州市科技计划项目(No.11A63030233)资助项目~~

摘　　要：该文用酶解一振荡法分离蝴蝶兰受精后的胚囊，然后显微解剖出合子、早期原胚及接近成熟的球形原胚。酶解液由O．7％-1．3％的纤维素酶、0．6％-1．0％果胶酶和10％甘露醇组成，pH5．8，酶解时间20-30min。分离的蝴蝶兰早期原胚和接近成熟的球形原胚均发现有发达的胚柄吸器。Zygotes, proembryos and maturing spherical proembryos were isolated from Phalaenopsis hybrid embryo sacs by the method of manual microdissection after a pretreatment of combinnating enzymatic mac- eration with shaking the ovules. The enzymatic maceration was under 0.7%-1.3% cellulase, 0.6%-1.0% pectinase, 10% mannitol, pH5.8 for about 20-30 min. Developed suspensor haustorium was discovered on the micro-dissect- ed embryos from three-cell stage to maturing spherical.

关 键 词：蝴蝶兰 合子 原胚 胚柄吸器 分离操作 

分 类 号：S682.31[农业科学—观赏园艺]