丛枝菌根真菌对枳根净离子流及锌污染下枳苗矿质营养的影响  

Effects of arbuscular mycorrhizal fungus on net ion fluxes in the roots of trifoliate orange(Poncirus trifoliata) and mineral nutrition in seedlings under zinc contamination

作　　者：肖家欣[1,2] 杨慧[1] 张绍铃[2] 

机构地区：[1]安徽师范大学生命科学学院,生物环境与生态安全安徽省高校省级重点实验室,芜湖241000 [2]南京农业大学园艺学院,南京210095

出　　处：《生态学报》2012年第7期2127-2134,共8页Acta Ecologica Sinica

基　　金：高等学校博士学科点专项科研基金项目(20103424120002);安徽省自然科学基金项目(070411004);安徽省高等学校省级自然科学研究项目(KJ2012A128);中国博士后科学基金项目(20080430172)

摘　　要：盆栽实验研究了不同施Zn水平(0、300 mg/kg和600 mg/kg)下,接种丛枝菌根真菌Glomus intraradices对枳苗生长、Zn、Cu、P、K、Ca、Mg分布的影响,并采用非损伤微测技术测定分析了菌根化与非菌根化枳根净Ca2+、H+、NO-3离子流动态。结果表明:(1)在不同施Zn水平下,接种菌根真菌显著提高了枳苗地上部及根部鲜重;随着施Zn水平的提高,菌根侵染率呈降低趋势,枳苗地上部与根部Zn含量呈增加趋势,且接种株根部Zn含量显著高于未接种株。(2)接种株未施Zn处理的地上部Cu、P、K、Mg和根部Cu含量、施600 mg/kg Zn处理的根部Cu及施300 mg/kg Zn处理的根部P含量均显著高于对照,而菌根真菌侵染对枳苗Ca含量并无显著性影响。(3)接种株未施Zn处理的根部距根尖端0μm和600μm处净Ca2+流出速率、600μm处净H+流入速率、2400μm处净NO-3流入速率均显著高于未接种株。We investigated the effects of arbuscular mycorrhizal fungus inoculation on net ion flux in the roots of trifoliate orange（Poncirus trifoliata（L.） Raf.） and mineral nutrition in seedlings under zinc contamination.A pot experiment was conducted to study plant growth as well as the distribution of zinc,copper,phosphorus,potassium,calcium,and magnesium in trifoliate orange seedlings inoculated by the arbuscular mycorrhizal fungus,Glomus intraradices,under greenhouse conditions.Plants were grown in yellow soil：quartz sand（9 ∶ 1,v/v） medium and were irrigated with solutions containing three different zinc concentrations（0,300 and 600 mg/kg）,which corresponded to control,medium and high zinc contamination treatments,respectively.Meanwhile,a non-invasive micro-test technique was used to determine the net fluxes of Ca2＋,H＋ and NO-3 along mycorrhizal and non-mycorrhizal roots of trifoliate orange seedlings grown in medium with no zinc added.Arbuscular mycorrhizal colonization significantly increased the fresh weight of shoots and roots exposed to different zinc levels.With increasing zinc concentrations,the mycorrhizal colonization percentage decreased,while the zinc levels in the shoots and roots progressively increased.Zinc concentrations in the roots were significantly higher in arbuscular mycorrhizal seedlings than in non-arbuscular mycorrhizal seedlings.The shoot/root ratios of the zinc translocation coefficient gradually decreased in arbuscular mycorrhizal seedlings with increasing zinc concentrations.This indicated that at medium or high levels of zinc contamination,a lot of zinc was immobilized in roots through the establishment of mycorrhizae.Therefore,phytotoxicity might be alleviated.Copper,phosphorus,potassium,and magnesium concentrations in shoots of plants grown in medium with no added zinc were significantly higher in arbuscular mycorrhizal seedlings than those in non-arbuscular mycorrhizal seedlings.Similarly,copper concentrations in the roots of plants in medium with 600 mg/kg of a

关 键 词：丛枝菌根真菌 枳 净离子流 锌 矿质营养 

分 类 号：S666[农业科学—果树学]