G—CSF或SCF体外刺激前后阵发性睡眠性血红蛋白尿症患者骨髓造血干细胞内磷酸化STAT5的表达  

Expression of phosphorylated STAT5 in bone marrow hematopoietic stem cells of patients with paroxysmal nocturnal hemoglobinuria before and after in vitro G-CSF or SCF stimulation

作　　者：丁少雪[1] 付蓉[1] 王红蕾[1] 张田[1] 阮二宝[1] 瞿文[1] 梁勇[1] 王国锦[1] 王晓明[1] 刘鸿[1] 吴玉红[1] 宋嘉[1] 王化泉[1] 邢莉民[1] 关晶[1] 王珺[1] 李丽娟[1] 邵宗鸿[1] 

机构地区：[1]天津医科大学总医院血液肿瘤科,300052

出　　处：《中华医学杂志》2012年第14期956-959,共4页National Medical Journal of China

基　　金：国家自然科学基金，卫生部卫生行业科研专项，中国医师协会课题，高等学校博士学科点专项科研基金，天津市自然科学基金，天津市科技支撑计划重点项目

摘　　要：目的观察阵发性睡眠性血红蛋白尿症（PNH）患者骨髓正常造血克隆（CD34＋CD59＋）和异常造血克隆（CD34＋CD59-）细胞在粒细胞集落刺激因子（G—CSF）或干细胞因子（SCF）体外刺激后胞质内信号转导及转录激活子5（STAT5）磷酸化表达差异。方法26例PNH患者及14名健康对照骨髓单个核细胞（BMMNC）经和不经100ng／mlG—CSF、SCF刺激，应用蛋白磷酸化流式细胞术分别检测其中CD34＋CD59＋和CD34＋CD59-细胞磷酸化STAT5（P—STAT5）的平均荧光强度（MFI）。结果（1）细胞因子刺激前，PNH患者CD34＋CD59-细胞内P—STAT5的MFI（单位：道数）为（24±18），明显低于CD34＋CD59＋细胞（644-49）和健康对照CD34＋CD59＋细胞（614-33）（均P〈0．01）；后两者间差异无统计学意义（P〉0．05）。（2）G—CSF刺激后，PNH患者CD34＋CD59-细胞P-STAT5的MFI为（36±35），明显低于CD34＋CD59＋细胞（124±84）和健康对照CD34＋CD59＋细胞（116±59）（均P〈0．01）；后两者间差异无统计学意义（P〉0．05）。（3）SCF刺激后，PNH患者CD34＋CD59-细胞P—STAT5的MFI为（34±27），明显低于CD34＋CD59＋细胞（124±97）和健康对照CD34＋CD59＋细胞（128±62）（均P〈0．01）；后两者间差异无统计学意义（P〉0．05）。（4）G—CSF或SCF刺激后，PNH患者及健康对照CD34＋CD59＋细胞P—STAT5的MFI较刺激前的增高程度，均明显超过PNH患者CD34＋CD59-细胞（均P〈0．01）。结论PNH克隆在体外应用G—CSF、SCF刺激后，胞内信号传导（STAT5）磷酸化明显低于正常造血克隆。Objective To study the expressions of phosphorylated STATS （P-STATS） in CD34 ＋ CD59- and CD34 ＋ CD59 ＋ bone marrow cells of the patients with paroxysmal nocturnal hemoglobinuria （PNH） before and after in vitro G-CSF or SCF stimulation, then evaluate the functions of G-CSF and SCF receptors in PNH clone ceils. Methods Bone marrow mononuelear cells （BMMNC） of 26 PNH patients and 14 normal controls were stimulated in vitro with G-CSF （100 ng/ml） or SCF （100 ng/ml） for 10 min. Before and after these stimulations, the mean fluorescence intensity （MFI） of P-STATS in CD34 ＋ CD59 ＋ BMMNC and CD34 ＋ CD59 - BMMNC were measured by flow cytometry. Results （ 1 ） The P-STATS MFI was （24 ± 18 ） in unstimulated CD34 ＋ CD59 - cells. And it was significantly lower than that in unstimulated CD34 ＋ CD59 ＋ cells of PNH patients （ 64 ± 49 ） and normal controls （ 61± 33 ） （ both P 〈 0.01 ）. No statistic difference existed between the latter two. （ 2 ） The P-STATS MFI was （ 36 ± 35 ） in G-CSF stimulated CD34 ＋ CD59 - cells of PNH patients. And it was significantly lower than that in G-CSF stimulated CD34 ＋ CD59 ＋ cells of PNH patients （ 124 ± 84） and normal controls （ 116 ± 59） （ both P 〈 0. 01 ）. There was no statistic difference between the latter two. （3）The P-STATS MFI was （34 ± 27） in SCF stimulated CD34 ＋ CD59 - cells of PNH patients. And it was significantly lower than that in SCF stimulated CD34 ＋ CD59 ＋ cells of PNH patients （ 124 ± 97 ） and normal controls （ 128 ±62 ） （ both P 〈 0. 01 ）. And no statistic difference existed between the latter two. （4）The increased P-STATS MFI in G-CSF stimulated CD34 ＋ CD59- cells of PNH patients was significantly lower than that in G-CSF stimulated ED34 ＋ CD59 ＋ cells of PNH patients and normal controls （ both P 〈 0. 01 ）. No statistic difference existed between the latter two. The increase of P- STATS MFI in SCF stimulated CD34 ＋ CD59 - cells of.PN

关 键 词：血红蛋白尿 阵发性 造血干细胞 粒细胞集落刺激因子 干细胞因子 STAT5 

分 类 号：R55[医药卫生—血液循环系统疾病]