大鼠GTH细胞PKC对FSH-β mRNA表达的影响  

Effects of PKC in Rats' GTH Cells on the Expression of FSH-β mRNA

作　　者：王新[1,2] 谭建华[3] 赖小平[2] 

机构地区：[1]青岛农业大学动物科学技术学院,山东青岛266109 [2]广州中医药大学中药学院,广东广州510006 [3]解放军军事医学科学院十一研究所,吉林长春130062

出　　处：《动物医学进展》2012年第4期45-48,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金(30571356);青岛农业大学高层次人才科研基金(630810);广州中医药大学博士后研究人员科研资助(B3YH1008)

摘　　要：为研究大鼠促卵泡素(FSH)分泌的受体后信号转导机制,将GTH细胞用PMA或H7处理后,用GnRH脉冲刺激,再用实时荧光定量PCR方法测定细胞FSH-βmRNA表达的Ct值,并与空白对照组比较。结果表明,PMA能显著降低GTH细胞FSH-βmRNA表达的Ct值,H7则曾能显著升高,说明GTH细胞PKC活性显著影响FSH-βmRNA的表达,FSH-βmRNA的表达随着PKC活性的升高而显著升高,随着PKC活性的降低而显著降低。因此,PKC-Ca2+是GnRH脉冲刺激所引起的FSH-βmRNA表达的受体后的信号转导途径。This article is to analyze the post-receptor signal transduction mechanism of the secretion of follicle stimulating hormone（FSH） in rats.The gonadotropic hormone（GTH） cells was stimulated by gonadotrophin hormone-releasing hormone（GnRH） after the activity of protein kinase C（PKC） in them was treated by phorbol 12-myristate 13-acetate（PMA） or 1（5-isoquinoline sulfonyl）-2-methyl piperazine dihydroch loride（H7）.Ct of FSH-β mRNA was detected by real time quantitative PCR（RTqPCR）,which was compared with the control group.The result showed that PMA can significantly reduce the Ct of FSH-β mRNA in GTH cells,while H7can significantly improveit.The activity of PKC in GTH cells influences the expression of FSH-β mRNA significantly,the expression of FSH-β mRNA rises with the rise of PMA and falls with the falling of H7.The conclusion is drawn that PKC-Ca ^2＋ is the post-receptor signal transduction pathway that the cells stimulated by GnRH impulse express FSH-β mRNA.

关 键 词：促性腺激素释放激素 促卵泡激素 蛋白激酶C 促性腺激素 信号转导 

分 类 号：S852.2[农业科学—基础兽医学]