实验性急性出血坏死性胰腺炎引发胰性脑组织损害及纳屈酮治疗作用  

The releationship between oxygen free radical and Phospholipase A_2 and therapeutic effect of Naltrexone on experimental pancreatic cerebral harm induced by AHNP in Rats

作　　者：赵海平[1] 王万祥[1] 刘淑平[2] 欧阳晓晖[1] 杨成旺[1] 孔广忠[1] 寿乃延[1] 

机构地区：[1]内蒙古医学院一附院普外科,呼和浩特010050 [2]内蒙古医学院生化教研室

出　　处：《肝胆胰外科杂志》2000年第2期93-95,共3页Journal of Hepatopancreatobiliary Surgery

摘　　要：目的 :探讨大鼠急性出血坏死性胰腺炎 (AHNP)引发胰性脑组织损害 (PCH)机理及评价纳屈酮治疗效果。方法 :应用 5 %牛磺胆酸钠逆行胰胆管注射诱发大鼠 AHNP并发脑组织损害模型。实验分为对照组、PCH组及纳屈酮 (NTX)治疗组 ;分别于 6、12、2 4h检测血浆及脑组织丙二醛 (MDA)、磷脂酶 A2 (PL A2 )、超氧化物歧化酶 (SOD)、胰腺和脑组织病理学改变。结果 :PCH组 6、12、2 4h血浆及脑组织较对照组 :MDA、PL A2 升高 ;SOD下降。 PCH组光镜下 6、12、2 4h分别出现脑组织水肿、点状出血、脱髓鞘改变。 NTX治疗组较 PCH组 :血浆及脑组织 MDA、PL A2 下降及 SOD升高 ;而且胰腺及脑组织损害减轻。结论 :大鼠 AHNP模型可引发 PCH;NTX可降低血浆及脑组织氧自由基、PL A2 ,同时增加 SOD,进而减轻脑组织损害。Objective: To study the releationship between oxygen free radical and Phospholipase A 2 and therapeutic effect of Naltrexone on experimental pancreatic cerebral harm(PCH) that was induced by AHNP in rats.Methods: A model of experimental PCH induced acute hemorrhagic necrotizing pancreatitis(AHNP) was induced by retrograde injection of 5% sodium taurocholate into the pancreatic duct.The rats were randomly divided into three groups:control group , PCH group and NTX group. We studied the effects of Naltrexone on the rats of PCH and measured the plasma and cerebral levels of malondialdenhyde(MDA),scavengers superoxidedismutase(SOD), Phospholipase A 2 . Changes of the pancreatic and cerebral histology were examined by light and electronic microscope. Results:In NTX treatment phase , the results demonstrated that MDA and PLA 2 fell significantly ,while SOD was increaced in the plasma and cerebral tissue and the damage of pancreatic and cerebral tissue were reduced.Conclusion:The experimental rat models is ideal to investigate PCH. NTX decreases oxygen free radicals and PLA_2 and improves the damage of cerebral and pancreatic tissue.

关 键 词：胰腺炎 脑组织损害 自由基 磷脂酶A2 治疗 

分 类 号：R657.51[医药卫生—外科学]