补肾益髓中药复方对糖皮质激素性骨质疏松症大鼠肾组织Osterix mRNA及蛋白表达的影响  

Effect of Reinforcing Kidney to Replenish Marrow Herbs Compound on mRNA and Protein Expression of Osterix in Kidney Tissues of Rats with Glucocorticoid-Induced Osteoporosis

作　　者：王剑 郑洪新[2] 刘研 张锦萍 刘瑞辉 

机构地区：[1]辽宁卫生职业技术学院,沈阳110101 [2]辽宁中医药大学基础医学院,沈阳110847

出　　处：《光明中医》2012年第4期673-677,共5页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：国家教育部博士点科研基金资助项目(No.200801620003)

摘　　要：目的观察糖皮质激素性骨质疏松症(GIOP)大鼠肾组织OsterixmRNA及蛋白表达,探讨GIOP的发病机制以及补肾益髓中药复方的疗效机理。方法肌注地塞米松复制GIOP大鼠模型,实验设正常组、模型空白组、补肾益髓中药复方组、补中益气颗粒组、血府逐瘀胶囊组、骨疏康颗粒阳性对照组。造模及灌胃给药9周。应用XR-26型双能X线骨密度仪测定股骨骨密度,实时定量RT-PCR及WesternBlot检测肾组织OsterixmRNA及蛋白表达。结果与正常组比较,模型空白组股骨骨密度明显降低(P<0.01)、肾组织OsterixmRNA及蛋白表达明显升高(P<0.01);与模型空白组比较,补肾益髓中药复方组股骨骨密度明显升高(P<0.01)、肾组织OsterixmRNA及蛋白表达明显下调(P<0.01)。结论肾组织OsterixmRNA及蛋白表达升高可能是GIOP的发病机制之一;补肾益髓中药复方可能通过下调肾组织OsterixmRNA及蛋白表达,有效防治GIOP。Objective To observe mRNA and protein expression of Osterix in kidney tissues of the rats with glucocorticoid-induced osteoporosis（GIOP） and discuss the pathogenesis of GIOP and the therapeutic effect and mechanism of reinforcing the kidney to replenish marrow herbs compound. Methods We established the GIOP rat models by intramuscular injection of dexamethasone. We divided all the rats into normal group, blank model group, reinforcing the kidney to replenish marrow herbs compound group, reinforcing the middle to benefit qi granula group,removing blood-house stasis capsule group and GUSHUKANG granula positive control group. After 9 weeks of establishing models and administrating by gavage, we detected femur bone mineral density by using the XR-26 dual energy X-ray absorptiometry. We detected mRNA expression of Osterix in kidney tissues by Real-Time Quantitative RT-PCR and detected their protein expression by Western Blot. Results Compared with normal group, femur bone mineral density of blank model group decreased significantly （P 〈 0. 01 ） , mRNA and protein expression of Osterix increased significantly（P 〈 0. 01 ） in kidney tissues ; Compared with blank model group, femur bone mineral density of reinforcing the kidney to replenish marrow herbs compound groupincreased significantly（ P 〈 0. 01 ） , mRNA and protein expression of Osterix down-regulated significantly（P 〈 0. 01 ） in kidney tissues. Conclusions Maybe it is one aspect of the pathogenesis of GIOP that mRNA and protein expression of Osterix increases in kidney tissues; Reinforcing the kidney to replenish marrow herbs compound can prevent and treat GIOP effectively by down-regulating mRNA and protein expression of Osterix in kidney tissues.

关 键 词：糖皮质激素性骨质疏松症 补肾益髓中药复方 OSTERIX 

分 类 号：R285.5[医药卫生—中药学]