ApoE基因敲除小鼠动脉粥样硬化早期血脂变化及主动脉超声生物显微镜成像分析  

The Change of Plasma Lipids in Apolipoprotein-e Knockout Mice at the Early Stage of Atherosclerosis and Ultrasound Characteristics of Aorta Using Ultrasound Biomicroscopy

作　　者：杨娅[1,2] 王艳红[1,2] 谢谨捷[1,3] 李嵘娟[1] 李治安[1] 王征[1] 秦彦文[4] 

机构地区：[1]首都医科大学附属北京安贞医院超声科,北京市100029 [2]首都医科大学附属北京友谊医院 [3]北京市朝阳区第二医院 [4]北京市心肺血管疾病研究所心血管生物研究室

出　　处：《中国超声医学杂志》2012年第4期304-307,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金(No.30672000);北京市自然科学基金(No.7102044和No.7082020)

摘　　要：目的分析ApoE基因敲除(ApoE—/—)小鼠血脂变化及主动脉动脉粥样硬化的超声生物显微镜(UBM)成像特征。方法 8周龄及16周龄雄性ApoE-/—小鼠各8只为实验组,同周龄同数量的雄性C57BL/6小鼠为对照组。应用超声生物显微镜观察小鼠主动脉根部、升主动脉和主动脉弓的形态结构,于主动脉根部短轴切面测量内-中膜厚度(IMT),并测定小鼠血脂。结果 ApoE—/—小鼠血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)及低密度脂蛋白(low density lipoprotein,LDL)水平较同周龄对照组明显升高(P<0.01);高密度脂蛋白(high density lipoprotein,HDL)水平较同周龄对照组减低(P<0.05)。16周龄ApoE-/—小鼠血脂较8周龄ApoE—/—小鼠增高更为明显,差异有统计学意义(P<0.01)。ApoE—/—小鼠的主动脉根部IMT较同周龄对照组增厚,差异有统计学意义(P<0.01);16周龄ApoE—/—小鼠IMT(0.208±0.038)mm较8周龄ApoE—/-小鼠主动脉根部IMT(0.121±0.025)mm增厚更为明显,差异有统计学意义(P<0.01)。结论 ApoE-/-小鼠早期出现高脂血症,UBM成像可显示小鼠主动脉早期动脉粥样硬化的病变特征。Objective The purpose of this study was to analyse the association between ultrasound biomicroscopy （UBM）characteristics and the change of plasma lipids in the Apolipoprotein E knockout （ApoE--/--） mice. Methods Two different age （8 weeks and 16 weeks ） groups of male ApoE /-- mice were used as atherosclerotic groups. Another 2 groups of age-matched male C57BL/6 mice were used as normal controls. Intima medial thickness was measured in the aortic root short-axis images by UBM. The concentrations of total cholesterol （TC）, triglycerides （TG）, high density lipoprotein cholesterol （HDL C） and low density lipoprotein cholesterol （LDL-C） were measured. Results Serum TC, LDL-C and TG were significantly higher in the ApoE--/-- groups than those in the age matched controls （P〈0.01）. HDLC was lower in the ApoE--/-- groups than that in the controls （P〈0.05） . IMTs in the aortic root were thicker in ApoE--/-- mice than those in the controls at same weeks of age. And the IMTs of 16 weeks were thicker than 8-week old ApoE--/-- mice（P〈0.01）. Conclusions At the early stage of atherosclerosis, ApoE--/-- mice appeared hypercholesteremia. UBM provides a noninvasive method of detecting early atherosclerotic progression in ApoE--/-- mice.

关 键 词：血脂 载脂蛋白E 小鼠 动脉粥样硬化 超声生物显微镜 

分 类 号：R543.5[医药卫生—心血管疾病] R445.1[医药卫生—内科学]