雷竹花芽形态分化过程中内源激素的变化规律  

Endogenous hormone changes during floral bud morphological differentiation of Phyllostachys violascens

作　　者：鲁亚婷[1] 袁晓亮[1] 林新春[1] 方伟[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《浙江农林大学学报》2012年第2期161-165,共5页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(31000295);国家重点基础研究发展计划("973"计划)项目(2012CB723008)

摘　　要：采用间接酶联免疫吸附分析法(ELISA)测定了雷竹Phyllostachys violascens花芽形态分化过程中的内源激素变化规律。结果表明:随着花芽形态分化程度的增加,吲哚乙酸(IAA)和异戊烯基腺苷(iPA)质量摩尔浓度表现为先迅速下降后又逐步回升到一稳定值;脱落酸(ABA)与赤霉素(GA)质量分数有一定波动,但大体上表现为先上升再下降再上升的趋势;ABA/IAA,GA/IAA和iPA/IAA均表现为先上升后下降的趋势,而且峰值均出现在花芽长度为12～14 mm的时期。此外,开花植株的iPA和ABA水平,ABA/IAA和iPA/IAA基本上高于未开花植株,而IAA和GA水平低于未开花植株,表明上述内源激素水平及比值对雷竹花芽形态分化有相应的促进与抑制作用。Endogenous hormones that occurred during flower bud morphological differentiation of the bamboo Phyllostachys violascens were determined by indirect enzyme-linked immunosorbent assay（ELISA）.Results showed that with development of flower bud morphological differentiation,the concentration of indoleacetic acid（IAA） and isopentenyladenosine（iPA） initially declined rapidly and then recovered gradually to a relatively stable value.Meanwhile,the concentration of abscisic acid（ABA） and gibberellin（GA） fluctuated but overall showed an up—down—up curve.The ABA/IAA,GA/IAA,and iPA/IAA values increased then decreased.In addition,the concentrations of iPA and ABA,as well as the ABA/IAA and iPA/IAA values for flowering bamboo were higher than vegetative bamboo with the concentrations of IAA and GA for flowering bamboo being lower than vegetative bamboo.Thus,flower bud morphological differentiation of Ph.violascens could be accelerated or inhibited by appropriate hormones.

关 键 词：植物学 雷竹 内源激素 花芽形态分化 花芽长度 

分 类 号：S718.3[农业科学—林学] Q945.4[生物学—植物学]