Identification of a NodD repressible gene adjacent to nodM in Rhizobium leguminosarum biovar viciae  

作　　者：Xiao'er Yang Bihe Hou Chenzhi Zong Guofan Hong 

机构地区：[1]State Key Laboratory of Molecular Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,ChineseAcademy of Sciences,Shanghai 200031,China

出　　处：《Acta Biochimica et Biophysica Sinica》2012年第4期323-329,共7页生物化学与生物物理学报（英文版）

摘　　要：The nodFEL and nodMNT operons in Rhizobium legumi- nosarum biovar viciae are transcribed in the same orien- tation and induced by NodD in response to flavonoids secreted by legumes. In the narrow intergenic region between nodFEL and nodMNT, we identified a small gene divergently transcribed from nodM to the 3＇ end of nodL. Unlike the promoters upstream of nodF and nodM, the promoter of this gene is constitutively expressed. It appeared that its promoter might partially overlap with that of nodM and its expression was repressed by nodD. A deletion mutation was made and proteins produced by the mutant were compared with those by wild-type using 2D gel electrophoresis. Several protein differences were identified suggesting that this small gene influences the ex- pression or stability of these proteins. However, the mutant nodulated its host plant （pea） normally.The nodFEL and nodMNT operons in Rhizobium legumi- nosarum biovar viciae are transcribed in the same orien- tation and induced by NodD in response to flavonoids secreted by legumes. In the narrow intergenic region between nodFEL and nodMNT, we identified a small gene divergently transcribed from nodM to the 3＇ end of nodL. Unlike the promoters upstream of nodF and nodM, the promoter of this gene is constitutively expressed. It appeared that its promoter might partially overlap with that of nodM and its expression was repressed by nodD. A deletion mutation was made and proteins produced by the mutant were compared with those by wild-type using 2D gel electrophoresis. Several protein differences were identified suggesting that this small gene influences the ex- pression or stability of these proteins. However, the mutant nodulated its host plant （pea） normally.

关 键 词：nodM px3 2D gel electrophoresis proteindifference 

分 类 号：Q911.631[天文地球—古生物学与地层学] Q939.114[生物学—古生物学]