急性咽结膜炎暴发流行的病毒病原学研究  

Viral etiology study of acute conjunctivitis

作　　者：何雅青[1] 赵锦铭[2] 杨洪[1] 严岚[2] 刘树强[2] 程红[2] 

机构地区：[1]深圳市卫生防疫站病毒科,518020 [2]首都儿科研究所病毒室

出　　处：《中华实验和临床病毒学杂志》2000年第1期40-43,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　探讨 1997年夏深圳地区暴发流行的急性咽结膜炎的病毒病原学。方法　采用急性咽结膜炎患儿急性期眼、咽拭子进行病毒分离 ,并同时用PCR法检查标本中病毒及分离病毒鉴定 ,对分离出部分毒株采用多个内切酶进行内切酶分析。此外 ,还测定患儿双份血中病毒的中和抗体增长。结果　 14份患儿咽拭子和 14份眼拭子病毒分离阳性率分别为 71 43%和 6 4 2 9% ;PCR法检测标本中病毒DNA的检出率分别为 78 5 7%和 71 43%。所有分离病毒株均为 3型腺病毒。选择其中 3对 (咽和眼拭子分离的毒株 )病毒株。内切酶分析显示均为Ad3a2基因型。 2 4例患儿双份血清均显示抗体效价≥ 4倍增长。分离株与其自身急性期和恢复期血清的中和试验也显示出恢复期中和抗体均较急性期有≥ 4倍增长。结论　本次急性咽结膜炎暴发流行是由 3型腺病毒引起。Objective To study the viral etiology of acute conjunctivitis broke out in Shenzhen in 1997. Methods Isolated the virus from eye and pharynx swabs of acute conjunctivitis in acute stage ,and detected virus nucleic acid by polymerase chain reaction(PCR). DNA of viral strains were subjected to restriction enzyme analysis using several endonucleases.Also,neutralizing antibody of paired sera were detected.Results The positive virus isolation rates of 14 pharynx and eye swabs were 71 43% and 64 29%,respectively;and that of the virus DNA by PCR were 78 57% and 71 43%, respectively.All of the strains isolated were adenovirus type 3. Three paired viral strains chosen and suabjected to restriction enzyme analysis proved belonging to Ad 3a 2 gene type. The neutralization test of paired sera of 24 cases showed that the antibody titers had 4 fold rising or above.Neutralization test of isolated viruses with paired sera of itself was carried out,the level of neutralizing antibody in convalescent atage was above 4 times as compared with that of acute stage.Conclusion The results showed that outbreak of acute conjunctivitis occured in 1997 in Shenzhen was caused by adenovirus type 3.

关 键 词：病毒性结膜炎 腺病毒 病毒抗体 PCR 

分 类 号：R511[医药卫生—内科学]