硫酸酯化箬叶多糖抗HIV-1机制的初步研究  

Mechanism of Se-Indocalamus tessdatus polycassine(S-ITPS) agacnst human immunodeficiency virus type 1(HIV-1)

作　　者：蒋岩[1] 陈春英[2] 裴丽健[1] 张辉[1] 魏文青[3] 王桂杰 邵一鸣 

机构地区：[1]卫生部艾滋病预防与控制中心参比实验室,北京100050 [2]中国科学院高能物理研究所化学室 [3]军事医学科学院放射医学研究所

出　　处：《中华实验和临床病毒学杂志》2000年第1期56-59,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　探讨硫酸脂化箬液多糖 (S ITPS)抗HIV的机制 ,为进一步开发该药提供理论依据。方法　于病毒接种前 ,接种同时及接种后分别在培养细胞 (MT 4)中加入硫酸脂化箬叶多糖 (S ITPS) ,在S ITPS存在或不存在的条件下培养 1～ 4周。终点以病毒半数感染量 (TCID50 法 ) ,细胞病变 (CPE) ,MTT染色细胞保护率 (MTT法 )及培养上清中的p2 4抗原滴度 (ELISA法 )作为评价指标 ,判断药效 ,分析抗病毒靶位。结果　S ITPS在细胞无毒浓度作用下 (<12 5 0 μg/ml)可以有效地保护病毒感染细胞 ,使其不产生病变 (IC50 =10 μg/ml) ,并能抑制病毒复制 (IC50 =15 6 μg/ml)。在S ITPS存在下产生的少量病毒的感染性较对照组显著降低。动态观察结果表明 :随着培养时间的延长 ,S ITPS对病毒复制的抑制作用也增强。在培养的第 1,2 ,3,4周 ,对病毒复制的抑制率分别为 73 7% ,6 3 5 % ,87 7%和 95 4%。实验还显示S ITPS可以抑制病毒吸附 ,但用药物预处理的细胞不能阻断病毒感染细胞。S ITPS对游离病毒也有灭活作用。结论　S ITPS的抗病毒作用靶位在于阻断病毒吸附和抑制感染细胞中病毒的复制。对细胞病变的抑制作用强于对抗原合成的抑制 ,提示它不仅对病毒蛋白质合成有抑制作用 ,而且对病毒及其蛋白的病毒效应 (如细胞病变 )Objective To study the mechanism of Se Indocalamus tessdatus polycassine(S ITPS) against HIV 1 and to provide a theoretical support for developing the anti HIV drugs.Method S ITPS was added to MT 4 cells before viral inoculation,at the same time of viral inoculation and after viral inoculation, then the cells were cultured with or without S ITPS for 1 4 weeks, finally, cytopathic effect(CPE),MTT staining method for viable cells (MTT assay),p24 antigen titer (ELISA),or infectivity(TCID 50 assay)of the cultural supernatants were used as markers to monitor the virus growth.Results S ITPS can inhibit HIV induced CPE(IC 50 =10μg/ml)and viral replication (IC 50 =156 μg/ml)in dose dependent manner.The virus infectivity in the presence of S ITPS was greatly decreased than that of the control.The virus inhibition was enhanced under the presence of noncytotoxic drug concentration of < 1 250 μg/ml in cell culture, inhibition of viral replication by S ITPS was 73 7%, 63 5%, 87 7%and 95 4% at week 1, week 2, week 3 and week 4 of cultured cells respectively. It can also inhibit absorption of HIV 1 to MT 4 cells, but no inhibition of HIV 1 was seen when MT 4 cells were pretreated with S ITPS. Virus can be inactivated by the agent also.Conclusion S ITPS is an potent anti HIV 1 agent in MT 4/HIV 1 cultural system,at least two mechanisms were in cluded:inhibition both of HIV 1 abborption to MT 4 cells and viral replication in HIV infected cells.S ITPS inhibition of CPE is stronger than that of p24 antigen production.

关 键 词：HIV-1 病毒抑制物 硫酸酯化箬叶多糖 

分 类 号：R965[医药卫生—药理学]