人血白蛋白包裹猪肺动脉脱细胞纤维组织方法的建立  

Studies on approaches of human albumin covering decellularized fiber tissue

作　　者：郭海平[1] 李温斌[2] 郭俊平[1] 靳振生[1] 薛丽霞[1] 崔鹤仙[1] 梁建国[1] 

机构地区：[1]邯郸市第一医院心胸外科,河北邯郸056002 [2]首都医科大学附属北京安贞医院心脏外科,北京100029

出　　处：《心脏杂志》2012年第2期181-184,共4页Chinese Heart Journal

摘　　要：目的:建立以人血白蛋白包裹脱细胞猪肺动脉纤维支架的方法。方法:采用自主研制的"脱水浸泡法"将人血白蛋白渗透至脱细胞猪肺动脉纤维支架内,比较脱细胞纤维组织和经白蛋白包裹的脱细胞纤维组织在物理性能方面的异同。通过免疫组织化学染色法评价白蛋白包裹脱细胞组织的效果。结果:采用"脱水浸泡法"能够使白蛋白有效地结合在脱细胞纤维组织内,完成人血白蛋白对脱细胞纤维组织的全面包裹。结论:采用"脱水浸泡法",人血白蛋白能够充分包裹脱细胞纤维支架,同时人血白蛋白包裹的脱细胞纤维组织的物理性能优于单纯脱细胞纤维组织。AIM： To observe the approach of human albumin coveting decellularized fiber tissue. METHODS： Human albumin was penetrated into decellularized porcine pulmonary fiber scaffolds by the method of ＂ dewatering and soaking. ＂ We next examined the similarities and differences of physical features between decellularized tissue and human albumin-decellularized tissue. The effects of human albumin covering decellularized tissue were evaluated by immunohistochemical methods. RESULTS： Using the ＂dewatering and soaking＂ method, human albumin could effectively combine with decellu- larized porcine pulmonary fiber scaffolds. Human albumin completely covered decellularized tissue. CONCLUSION： Using the ＂dewatering and soaking＂ method, human albumin may effectively combine with decellularized porcine pulmonary fiber scaffolds. Human albumin-decellularized tissue was superior in physical features to decellularized tissue.

关 键 词：白蛋白 组织工程 心脏瓣膜 肺动脉 猪 

分 类 号：Q512.1[生物学—生物化学]