Ammonium-dependent regulation of aerobic methane-consuming bacteria in landfill cover soil by leachate irrigation  

作　　者：Fan Lü Pinjing He Min Guo Na Yang Liming Shao 

机构地区：[1]State Key Laboratory of Pollution Control and Resources Reuse,College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China

出　　处：《Journal of Environmental Sciences》2012年第4期711-719,共9页环境科学学报（英文版）

基　　金：supported by the National Basic Research Program (973) of China (No. 2012CB719801);the Program of Shanghai Subject Chief Scientist (No.10XD1404200);the Shanghai Pujiang Program (No.11PJ1409200)

摘　　要：The impacts of landfill leachate irrigation on methane oxidation activities and methane-consuming bacteria populations were studied by incubation of landfill cover soils with leachate and （NH4）2SO4 solutiort at different ammonium concentrations. The community structures and abundances of methane-oxidizing bacteria （MOB） and ammonia-oxidizing bacteria （AOB） were examined by PCR- DGGE and real-time PCR. Compared with the pure （NH4）2SO4 solution, leachate addition was found to have a positive effect on methane oxidation activity. In terms of the irrigation amount, ammonium in leachate was responsible for the actual inhibition of leachate. The extent of inhibitory effect mainly depended on its ammonium concentration. The suppression of the predominant methane- consuming bacteria, type I MOB, was responsible for the decreased methane oxidation activity by ammonium inhibition. Methane- consuming bacteria responded diversely in abundance to ammonium. The abundance of type I MOB decreased by fivefold; type II MOB showed stimulation response of fivefold magnification upon the first addition but lessened to be lower than the original level after the second addition; the amount of AOB was stimulated to increase for 20-30 times gradually. Accumulated nitrate from nitrification strengthened the ammonium inhibition on type I and type II MOB, as a result, repetitive irrigation was unfavorable for methane oxidation.The impacts of landfill leachate irrigation on methane oxidation activities and methane-consuming bacteria populations were studied by incubation of landfill cover soils with leachate and （NH4）2SO4 solutiort at different ammonium concentrations. The community structures and abundances of methane-oxidizing bacteria （MOB） and ammonia-oxidizing bacteria （AOB） were examined by PCR- DGGE and real-time PCR. Compared with the pure （NH4）2SO4 solution, leachate addition was found to have a positive effect on methane oxidation activity. In terms of the irrigation amount, ammonium in leachate was responsible for the actual inhibition of leachate. The extent of inhibitory effect mainly depended on its ammonium concentration. The suppression of the predominant methane- consuming bacteria, type I MOB, was responsible for the decreased methane oxidation activity by ammonium inhibition. Methane- consuming bacteria responded diversely in abundance to ammonium. The abundance of type I MOB decreased by fivefold; type II MOB showed stimulation response of fivefold magnification upon the first addition but lessened to be lower than the original level after the second addition; the amount of AOB was stimulated to increase for 20-30 times gradually. Accumulated nitrate from nitrification strengthened the ammonium inhibition on type I and type II MOB, as a result, repetitive irrigation was unfavorable for methane oxidation.

关 键 词：greenhouse gas emission landfill leachate methane oxidation methane-oxidizing bacteria （MOB） ammonia-oxidizing bacteria （AOB） 

分 类 号：S572[农业科学—烟草工业] X705[农业科学—作物学]