两株脂肪类混合细菌拆分缩水甘油丁酸酯的工艺研究  

Resolution of Glycidyl Butyrate with Two Mixed and Screened Alkaline Lipase

作　　者：黄强[1] 孟庆意[1] 牛柏林[1] 张蕊[1] 高莹玉[1] 

机构地区：[1]郑州大学化工与能源学院,河南郑州450001

出　　处：《郑州大学学报（工学版）》2012年第2期36-40,共5页Journal of Zhengzhou University（Engineering Science）

基　　金：河南省自然科学基金资助项目(082300440040)

摘　　要：由于从土壤筛选出十几个单株细菌拆分效果不理想,从中优选出两株脂肪类细菌混合使用,拆分效果良好.其选择性拆分外消旋体缩水甘油丁酸酯的工艺为:淀粉为碳源,牛肉膏为氮源,初始pH=8.0,温度30℃,转速200 r/min,摇瓶2 d,产酶量为1.5 U.在最适条件下培养两株细菌拆分反应,并考察工艺条件对拆分反应效果的影响.结果表明,工艺条件在温度28℃,pH=6.0,30 mL脂肪类双菌液中加入0.5 g缩水甘油丁酸酯,在200 r/min下振荡12 h,拆分效果良好,(R)-缩水甘油丁酸酯的对映体过量值(ee)为92.8%,转化率为83.2%.Two mixed strains of microorganism capable of optically resolving racemic glycidyl butyrate have been isolated from soil and identified to be alkaline lipase,which one strain of lipase has no ideal result from tens of lipase.The suitable carbon source of the condition was starch and the nitrogen source was beaf extract.The proper temperature and initial pH for enzme formation were 30 ℃ and pH=8.0 respectively.Rotating rate was 200 r/min.Under two days fermentation,it can reach 1.5 u/mL.The technology of the two alkaline lipase in the optical resolution of glycidyl butyrate was studied.The condition was pH 6.0,28 ℃,200 r/min,0.50 g substrate added in the alkaline lipase liquid,when the glycidyl butyrate was transformed by 83.2%,the optical purity of（R）-glycidyl butyrate was 92.8% ee（enantiomeric excess）.

关 键 词：缩水甘油丁酸酯 光学拆分 脂肪类细菌 

分 类 号：TQ225.24[化学工程—有机化工] TQ326