2010-2011年广东省东莞市甲型H1N1流行性感冒病毒血凝素基因变异特征研究  

Genetic characteristics of hemagglutinin of Influenza A(H1N1) pdm 09 virus in Dongguan city in Guangdong province,2010-2011

作　　者：黄勇[1] 杨华可[1] 袁达康[1] 黎景全[1] 李艳芬[1] 周建孟[1] 李宇政[1] 陈永迪[1] 

机构地区：[1]东莞市疾病预防控制中心,广东东莞523129

出　　处：《疾病监测》2012年第3期195-199,共5页Disease Surveillance

摘　　要：目的了解2010-2011年广东省东莞市甲型H1N1流行性感冒(流感)病毒血凝素(HA)基因的特征及变化。方法反转录-聚合酶链反应(RT-PCR)、基因扩增、测序得到标本HA基因的核酸序列,利用生物信息软件对序列的特征及变化情况进行分析。结果通过测序得到各标本HA基因1701 bp长度的核苷酸序列,566个氨基酸。2年间有24个氨基酸位点发生变异,但其中有12个位点的改变只出现在单个毒株上。发生明显变化趋势的位点只有145、202、391、468,其中202位点属于Sb抗原决定簇区。受体结合位点、潜在糖基化位点及二硫键比较稳定,2年间没有发生变化。结论主要功能区域比较保守,无变化。序列中发生明显变化趋势的位点只有4个,其中1个位于抗原决定簇区,属于流感病毒抗原漂移过程中突变的累积。Objective To understand the genetic characteristics of hemagglutinin（HA） of Influenza A（H1N1） pdm 09（H1N1） virus in Dongguan during 2010-2011.Methods The nucleotide sequences of HA gene of（H1N1） viruses were obtained by using RT-PCR and gene sequencing techniques.The characteristics of HA sequences were analyzed with bioinformatics software.Results The length of the nucleotide sequence of HA gene obtained by sequencing was 1701 bp and its corresponding amino acid sequence length was 566aa.During this period the mutations of 24 animo acid sites were found,among them 12 mutation sites were in single virus strain.Only 145,202,391 and 468 sites had obvious change trend,among them 202 sites was at Sb antigen epitope.The receptor binding locus,potential glycosylation site and disulfide bond were relatively stable,no change occurred.Conclusion The main functional region was conserved,no change was observed.Only four sites in all HA sequences had obvious change trend,1 located at antigen epitopes,which was the result of the mutation accumulation in antigen drift of influenza virus.

关 键 词：流感病毒 血凝素基因 变异 

分 类 号：R373[医药卫生—病原生物学]