Ghrelin在食管腺癌、Barrett's食管黏膜中的表达  

Ghrelin expression in esophageal adenocarcinoma and Barrett's esophagus

作　　者：甄云飞[1] 曲海霞[1] 王青[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院消化科(东院),山东省青岛市266071

出　　处：《世界华人消化杂志》2012年第9期781-783,共3页World Chinese Journal of Digestology

摘　　要：目的:研究Ghrelin在食管腺癌、Barrett's食管(barrett esophagus,BE)和正常食管黏膜中的表达.方法:选取30例食管腺癌患者、35例BE患者及35例健康对照,所有受试者均行胃镜检查,记录内镜下表现,在食管部位四象限活检取材,标本经10%甲醛固定,石蜡包埋,连续切片,分别用于改良HE染色及免疫组织化学,采用免疫组织化学方法检测Ghrelin在食管黏膜组织中的表达水平.结果:Ghrelin在食管腺癌组食管黏膜中的表达水平低于健康对照组和BE组,在BE组食管黏膜中的表达高于健康对照组,差异有统计学意义(1.34±0.51vs4.86±0.82vs3.54±0.79,F=27.21,P<0.05).食管腺癌组中,Ghrelin在中高分化腺癌患者食管黏膜的表达水平高于低分化腺癌,差异有统计学意义(Z=4.60,P<0.05).结论:Ghrelin在BE、食管腺癌黏膜中的表达不同,提示了Ghrelin在食管癌变前和癌变后的变化.食管腺癌组织恶变过程中其Ghrelin的分泌机制发生了改变,且与食管腺癌细胞分化程度有关.AIM：To investigate the expression of Ghrelin in the esophageal mucosa of patients with esophageal adenocarcinoma and those with Barrett＇s esophagus（BE）.METHODS：Thirty patients with esophageal adenocarcinoma,35 patients with BE,and 35 normal controls were enrolled in the study.The expression of Ghrelin in specimens taken from the above subjects was detected by immunohistochemistry.RESULTS：The expression of Ghrelin in esophageal adenocarcinoma was lower than that in normal controls and patients with BE.The expression of Ghrelin in the BE group was higher than that in the control group（1.34 ± 0.51 vs 4.86 ± 0.82 vs 3.54 ± 0.79,F = 27.21,P 0.05）.In the esophageal adenocarcinoma group,the expression of Ghrelin in moderately and well differentiated specimens were higher than that in poorly differentiated specimens（Z = 4.60,P 0.05）.CONCLUSION：The expression of Ghrelin in the esophageal adenocarcinoma is different from that in BE.The level of Ghrelin changes during the evolution of esophageal cancer.Disruption of the esophageal Ghrelin-producing mechanism may occur during esophageal carcinogenesis.There is an association between the degree of esophageal carcinoma differentiation and Ghrelin production.

关 键 词：食管腺癌 Barrett's食管 GHRELIN 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]