低分子肝素对伴高脂血症急性坏死性胰腺炎大鼠PAF、ET-1/NO的影响  

Treatment with low-molecular-weight heparin reduces blood levels of PAF and ET-1/NO in rats with severe acute pancreatitis and hyperlipemia

作　　者：王湘英[1] 卢义展 张兴文[3] 骆永富[1] 王强[1] 

机构地区：[1]湖南省人民医院肝胆外科,湖南省长沙市410005 [2]湖南省湘西自治州人民医院普外科,湖南省吉首市416000 [3]湖南省人民医院急诊科,湖南省长沙市410005

出　　处：《世界华人消化杂志》2012年第10期856-860,共5页World Chinese Journal of Digestology

基　　金：长沙市科技计划基金资助项目;No.k0803152-31~~

摘　　要：目的:研究低分子肝素(LMWH)治疗伴高脂血症性急性胰腺炎(HL-AP)大鼠微循环障碍的作用.方法:SD大鼠80只,高脂饲料喂养4wk建立高脂血症模型后,随机分成3组:假手术(S)组,n=16;NS对照(N)组,n=32;低分子肝素(L)组,n=32.N组与L组大鼠建立ANP模型,S组大鼠仅开腹翻动胰腺.L组造模后分别于0h,6h,12h及18h开始使用LMWH皮下注射.N、S组同时点同方法用生理盐水对照,24h后抽门静脉检测血小板活化因子(PAF)、内皮素-1(ET-1)/一氧化氮(NO)浓度并行电镜观察胰腺组织微循环结构.结果:L组PAF、ET-1/NO浓度分别为5.9250mmol/L±0.6113mmol/L及3.5368±0.26;N组为7.4059mmol/L±0.4281mmol/L及4.1697±0.08;SO组为4.4950mmol/L±0.2628mmol/L及2.5133±0.20;L组PAF、ET-1/NO浓度均低于N组但高于SO组,有统计学意义;L组中0h及6h开始用LMWH的大鼠PAF、ET-1/NO浓度低于12h及18h组,有统计学意义.电镜观察L组胰腺病变程度轻于N组.结论:高脂血症急性胰腺炎大鼠存在胰腺微循环障碍;LMWH可以改善高脂血症大鼠SAP胰腺微循环障碍,并且早期应用优于晚期应用.AIM： To investigate the effect of treatment with low-molecular-weight heparin （LMWH） on microcirculation dysfunction in rats with severe acute pancreatitis （SAP） and hyperlipemia. METHODS： SD rats were fed a high-fat diet for four weeks to induce hyperlipemia and ran-domly divided into sham operation group, control group, and experimental group. The control group and experimental group were then used to induce SAP, while the sham operation group underwent a sham operation. At 0, 6, 12 and 18 h after SAP induction, the experimental group was subcutaneouly injected with LMWH, and the control group was injected with normal saline. The concentrations of PAF and ET-1/NO in the portal vein blood were measured, and pancreatic microcirculatory structure was observed by electron microscopy. RESULTS： The concentrations of PAF and ET-1/NO in the experimental group （5.9250 mmol/L ± 0.6113 mmol/L, 3.5368 ± 0.26） were significantly lower than those in the control group （7.4059 mmol/L ± 0.4281 mmol/L, 4.1697 ± 0.08）, but were significantly higher than those in the sham operation group （4.4950 mmol/L ± 0.2628 mmol/L, 2.5133 ± 0.20）. In addition, treatment with LWMH improved pancreatic microcirculation. CONCLUSION： Treatment with LMWH improves pancreatic microcirculation in rats with SAP and hyperlipemia.

关 键 词：低分子肝素 高脂血症 重症急性胰腺炎 微循环 

分 类 号：R589.2[医药卫生—内分泌]