摇瓶培养条件下桑木层孔菌不同培养期多糖含量动态积累规律研究  

Study on polysaccharides accumulation in different growth phase of Phellinus mori under shake-flask culture condition

作　　者：张梅梅[1] 郑维发[1] 赵艳霞[1] 魏志文[1] 

机构地区：[1]徐州师范大学 江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《徐州师范大学学报（自然科学版）》2012年第1期65-68,共4页Journal of Xuzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31070052);江苏省自然科学基金资助项目(BK2010027;BK2009084);徐州师范大学自然科学基金资助项目(10XLA21)

摘　　要：采用滤纸片法测定菌丝体生物量以及苯酚-硫酸法测定不同培养期的桑木层孔菌多糖含量和发酵液中残糖含量,研究了摇瓶培养条件下不同培养期的桑木层孔菌多糖含量的动态积累规律.结果表明,桑木层孔菌在摇瓶中培养12d时,获得的菌丝体最多,干重达13.580g/L;培养8d时得到最多的胞外、胞内多糖,含量分别达4.585g/L和32.465mg/g;培养到12d时发酵液中残余糖量最少,为4.744g/L.该实验为进一步研究和开发利用桑木层孔菌多糖奠定了实验基础和科学依据.The polysaccharides accumulation of Phellinus mori in shake-flask culture is studied.Filter method was used to determine the mycelium biomass.Phenol-sulfuric acid method was used to determine the content of total polysaccharides.The result shows that the most Phellinus mori mycelia was got after 12 d,its dry weight was 13.580 g/L.After 8 d,the content of exopolysaccharides was 4.585 g/L and the content of inopolysaccharides was 32.465 mg/g.The least residual sugar was left after 12 d.The content of residual sugar was 4.744 g/L.The method for the further discussion and utilization of Phellinus mori laid the experiment foundation and the scientific basis.

关 键 词：桑木层孔菌 摇瓶培养 多糖 积累 

分 类 号：Q914.83[天文地球—古生物学与地层学]