抗氧化剂和NF_κB对大鼠肺微血管内皮细胞iNOS表达的影响  

Effect of antioxidant and NF_κB on the induction of iNOS gene in rat pulmonary microvascular endothelial cells in vitro

作　　者：刘清行[1] 金惠铭[1] 吴朝辉 陈愉[1] 刘凯 

机构地区：[1]上海医科大学病理生理教研室,上海200032

出　　处：《中国病理生理杂志》2000年第1期20-23,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨NFκB在大鼠肺微血管内皮细胞iNOS基因诱导表达中的作用及抗氧化剂对内皮细胞iNOS诱导表达的影响及其机制。方法 :Griess法测定细胞培养上清液NO-2 水平以反映NO的生成 ,Northern印迹分析iNOSmRNA水平 ,EMSA法测定细胞核内NFκB的结合活性。结果 :抗氧化剂可阻断内皮细胞培养体系LPS和TNFα诱导的NO生成及iNOSmRNA的表达 ;LPS和TNFα可诱导大鼠肺微血管内皮细胞NFκB的激活并可被抗氧化剂阻断。结论 :LPS和TNFα诱导的内皮细胞iNOS基因表达依赖于NFκB的激活 ;AIM:To investigate the role of nuclear factor κB (NF κB) in the induction of iNOS gene by TNFα and LPS in endothelial cells and the effect of antioxidant on the induction of iNOS. METHODS:Nitrite was determined based on Griess reaction. iNOS mRNA was analyzed using Northern blot. NF κB in the cell nucleole was detected with electrophoretic mobility shift assay.RESULTS:(1)NO production and iNOS mRNA expression induced by LPS and TNFα was blocked by pyrrolidine dithiocarbamate(PDTC) or N-acetylcysteine(NAC). (2)LPS and TNFα triggered the activation and translocation of NF κB, and PDTC or NAC inhibited the activation of NF κB induced by LPS and TNFα.CONCLUSIONS:(1)The induction of iNOS gene by TNFα and LPS is dependent on the activation of NF κB.(2)Antioxidants may inhibit the induction of iNOS gene through the inhibition of NF κB activation.

关 键 词：肺微血管 抗氧化剂 NFкB 一氧化氮合酶 

分 类 号：R543.02[医药卫生—心血管疾病]