左肾静脉不全结扎对大鼠肾水通道蛋白1和水通道蛋白2 mRNA表达的影响  

Effects of left renal vein partial ligation on rat renal aquaporin 1 and aquaporin 2 mRNA expression

作　　者：王蔚东[1] 田长生[1] 李杨[1] 李春凌[1] 赵丹[1] 赵雪俭[1] 杨宝学 

机构地区：[1]白求恩医科大学病理生理教研室,吉林长春130021 [2]美国加利佛尼亚大学

出　　处：《中国病理生理杂志》2000年第1期54-56,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :观察左肾静脉不全结扎大鼠肾AQP1和AQP2mRNA表达的变化 ,探讨肾静脉压增高引起肾小管重吸收减少的分子机制。方法 :成年雄性Wistar大鼠随机分成左肾静脉不全结扎组 (VCG)和假手术组(SOG) ,用左肾静脉不全结扎法复制精索静脉曲张 ,2月后进行Northernblot分析。结果 :与SOG相比 ,VCG大鼠左肾AQP1mRNA表达强度明显减弱 ,AQP2mRNA表达强度则无明显差异 ,睾丸组织无杂交信号。结论 :左肾静脉不全结扎大鼠肾静脉压增高引起肾小管重吸收减少可能与AQP1mRNA表达减少有关 。AIM:Changes of rat renal aquaporin (AQP1) and AQP2 mRNA expression after partially ligating left renal vein were observed to explore molecular mechanism of renal tubular reabsorption decrease resulted by increasing renal vein pressure.METHODS:Adult male rats were randomly divided into left renal vein partial ligation group(ie. VCG, varicocele group ) and sham operation group (SOG), left renal mRNA expression was analyed by Northen blot two months later.RESULTS:Intensity of left renal AQP1 mRNA expression in VCG was weaker than that in SOG, there was not any difference in left renal AQP2 mRNA expression between two groups, no hybridization signal was found in testes.CONCLUSION:Decrease of renal tubular reabsorption resulted by partially ligating renal vein might be related to decrease of AQP1 mRNA expression; “hormone escape" might occur in collecting tubules.

关 键 词：大鼠 肾静脉压 水通道蛋白 mRNA 

分 类 号：R692.02[医药卫生—泌尿科学]