2,3-丁二酮单肟对冷停搏心脏的保护作用  

Effect of 2,3-butanedione monoxime (BDM) on myocardial protection in cold cardioplegia

作　　者：欧阳静萍[1] 刘永明[1] 郑汉巧[1] 魏蕾 涂淑珍[1] 杨海鹭[1] 崔冶建[2] 

机构地区：[1]湖北医科大学病理生理学教研室,湖北武汉430071 [2]湖北医科大学实验检测中心,湖北武汉430071

出　　处：《中国病理生理杂志》2000年第1期70-73,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :观察心肌兴奋收缩脱敏剂 2 ,3-丁二酮单肟 (BDM)对冷停搏心脏的保护作用。方法 :本实验采用SD大鼠 48只 ,高K+ 组 (n =2 4)取心脏用StThomas液 4℃保存 ;BDM组 (n =2 4)用BDM -K -H液保存心脏。分别保存 8h ,18h ,2 4h后悬挂于Langendorff灌流装置 ,再灌注 40min。结果 :18h后 ,高K+ 组 6 0 %不复跳 ,心率和冠脉流量都显著低于BDM组 (P <0 0 5 ) ,心肌质膜和线粒体Na+ -K+ -ATPase活性也显著低于BDM组 (P <0 0 1) ;保存 2 4h后 ,高K+ 组心脏 10 0 %不复跳 ,BDM组全部复跳。结论 :BDM可以节省能量 。AIM: The effects of BDM on isolated rat heart in cold cardioplegia were studied METHODS: Rat heart were subjected to cold cardioplegia at 4℃ for 8, 18 and 24 h Then each heart was perfused (90 cm H 2O) in Langendorff model at 37℃ for 40 min In the high K + group( n =24) the hearts were preserved in St Thomas cardioplegic solution, in BDM group( n =24) hearts were preserved in K-H solution with BDM 30 mmoL/L RESULTS: After 18 h, heart rate and the coronary flow in BDM group were significantly higher than in high K + group( P <0 05) Activity of Na +-K +-ATPase in cell membrane and in mitochondrial membrane in the BDM group was significantly higher than high K + group( P <0 01) After 24 h, all hearts in high K + group were dead, but were alive in BDM group CONCLUSION: Under given experiment conditions, BDM did enhance the tolerance to cold ischemia significantly The results showed that BDM may become a useful agent for prolong the storage period of heart in cold cardioglegia

关 键 词：心脏停搏 心肌收缩 大鼠 BDM 冷停搏液 

分 类 号：R654.2[医药卫生—外科学] R318.52[医药卫生—临床医学]