日本血吸虫组织蛋白酶BDNA疫苗抗生殖免疫的研究  

STUDIES ON ANTI-FECUNDITY INDUCED BY CATHEPSIN B DNA VACCINE OF SCHISTOSOMA JAPONICUM

作　　者：易新元[1] 曾宪忠[2] 曾宪芳[1] 汪世平[1] 舒新华[1] LarryMcreynolds 

机构地区：[1]湖南医科大学寄生虫学教研室,长沙410078 [2]青岛大学医学院寄生虫学教研室,青岛266021 [3]NewEnglandBiolabs,MA,USA

出　　处：《中国人兽共患病杂志》2000年第1期45-47,共3页Chinese Journal of Zoonoses

基　　金：湖南省科委资助!(98ssy07) ;TDR/ WHO 资助!(980268)

摘　　要：目的　探讨日本血吸虫组织蛋白酶ＢＤＮＡ疫苗的保护。方法　分别用Ｓｊ３１ＢＩＮ免疫昆明鼠和ＢＡＬＢ／ｃ 小鼠，攻击感染后６ｗ 计数成虫负荷及粪便和组织内虫卵数。结果　用构建的Ｓｊ３１ＢＩＮ免疫小鼠可影响成虫发育，肝组织减卵率为５９－３％～６５－０％ ，肠组织减卵率为５７－６％ ～６２－１％ ，粪便减卵率为８６－５％ 。结论　Ｓｊ３１ＢＩＮ 可诱导小鼠产生抑制雌虫生殖的免疫力。Aim To study the anti-fecundity immunity effect in mice induced by Schistosoma japonicum cathepsin B DNA vaccine(Sj31BIN) Methods The DNA Vaccine of S japonicum was constructed by inserting Sj 319 one into vector VR 1012 The mice were immunized with Sj31BIN and the anti-fecundity immunity was determined by counting worms,and egg burden in liver,intestine and faeces on the 46th day after challenge infection with carcariae of S japonicum Results The immnization with Sj31BIN reduced the worm discovery and egg burden in tissues and faeces The egg reduction rate was 59 3%～65 0% in the liver,57 6%～62 1% in the intestine and 86 5% in the faeces ln addition,the number of eggs in the uteri of female adults was obviously decreased in mice immuicized with Sj31BIN,Conclusion Sj31BIN immunization induces anti-fecundity immunity in mice

关 键 词：日本血吸虫 组织蛋白酶B DNA疫苗 抗生殖系统 

分 类 号：R532.21[医药卫生—内科学]